Abstract
Salmonellosis caused by non-typhoid Salmonella serotypes is one of the most common causes of food-borne illness throughout the world. The diagnosis is primarily by culture and more recently molecular methods, whereas the use of serological methods for diagnosis of Salmonella infections is limited by high running costs as well as low sensitivity and specificity. Fast and reliable immunoassays for detection of S. typhi subunit antigens are commercially available, but there is no international consensus of similar tests for non-typhoid salmonellosis. Most immunoassays for non-typhoid human Salmonella diagnosis are developed in-house and used in-house for research or regional surveillance purposes. Only few laboratories use serology for the diagnosis of Salmonella-associated complications such as arthritis. Considering the current burden of disease, the development of a validated and standardized, commercially available antibody assay for diagnosing non-typhoid human salmonellosis can be of great benefit for diagnostic and surveillance purposes throughout the world.
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Acknowledgements
Acknowledgements are due to Tine Dalby and Charlotte Sværke Jørgensen at Statens Serum Institut, Denmark, for invaluable help with developing and validating the Salmonella ELISA protocol.
The work was partly financially supported by The European Centre for Disease Prevention and Control (framework contract ECDC/09/032) and the EU Network of Excellence for research on the prevention and control of zoonoses MEDVETNET (Contract no. FOOD-CT-2004-506122).
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Kuhn, K.G., Emborg, HD., Krogfelt, K.A., Mølbak, K. (2015). Detecting Non-typhoid Salmonella in Humans by Enzyme-Linked Immunosorbent Assays (ELISAs): Practical and Epidemiological Aspects. In: Schatten, H., Eisenstark, A. (eds) Salmonella. Methods in Molecular Biology, vol 1225. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1625-2_8
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DOI: https://doi.org/10.1007/978-1-4939-1625-2_8
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