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Live Cell Imaging of Intracellular Salmonella enterica

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Salmonella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1225))

Abstract

During the intracellular phase of the pathogenic lifestyle, Salmonella enterica massively alters the endosomal system of its host cells. Two hallmarks are the remodeling of phagosomes into the Salmonella-containing vacuole (SCV) as a replicative niche, and the formation of tubular structures, such as Salmonella-induced filaments (SIFs). To study the dynamics and the fate of these Salmonella-specific compartments, live cell imaging (LCI) is a method of choice. In this chapter, we compare currently used microscopy techniques and focus on considerations and requirements specific for LCI. Detailed protocols for LCI of Salmonella infection with either confocal laser scanning microscopy (CLSM) or spinning disk confocal microscopy (SDCM) are provided.

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Acknowledgements

We thank all the members of our laboratory for fruitful discussion and feedback especially Viktoria Krieger for additional hints for FuGENE TF and the CLSM system. Furthermore, we thank Rainer Kurre at CALMOS for constant and invaluable support of our microscope systems. Work was supported by Deutsche Forschungsgemeinschaft (DFG) through grants HE1964 and SFB944, project P4, and the Bundesministerium für Bildung und Forschung (BMBF).

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Authors and Affiliations

  1. Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany

    Alexander Kehl & Michael Hensel

Authors
  1. Alexander Kehl
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  2. Michael Hensel
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Correspondence to Michael Hensel .

Editor information

Editors and Affiliations

  1. Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA

    Heide Schatten

  2. Cancer Research Center, University of Missouri, Columbia, Missouri, USA

    Abraham Eisenstark

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Kehl, A., Hensel, M. (2015). Live Cell Imaging of Intracellular Salmonella enterica . In: Schatten, H., Eisenstark, A. (eds) Salmonella. Methods in Molecular Biology, vol 1225. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1625-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-1625-2_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1624-5

  • Online ISBN: 978-1-4939-1625-2

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