Abstract
The ability to isolate primary chondrocytes from wild-type and genetically altered mice has provided tremendous advances in the understanding of signaling networks that regulate chondrocytes in health and disease. Isolation of chondrocytes from both growth plate tissues and articular cartilage has been challenging due to the cells being embedded within a highly organized tissue matrix. Here we describe highly reproducible methods for the isolation of pure populations of growth plate chondrocytes from the murine sternum and ribs and articular chondrocytes from the knee joint.
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References
Wu JJ, Weis MA, Kim LS et al (2010) Type III collagen, a fibril network modifier in articular cartilage. J Biol Chem 285:18537–18544
Han EH, Chen SS, Klisch SM et al (2011) Contribution of proteoglycan osmotic swelling pressure to the compressive properties of articular cartilage. Biophys J 101:916–924
Zuscik MJ, Hilton MJ, Zhang X et al (2008) Regulation of chondrogenesis and chondrocyte differentiation by stress. J Clin Invest 118:429–438
Dao DY, Jonason JH, Zhang Y et al (2012) Cartilage-specific beta-catenin signaling regulates chondrocyte maturation, generation of ossification centers, and perichondrial bone formation during skeletal development. J Bone Miner Res 27:1680–1694
Kung MH, Yukata K, O’Keefe RJ et al (2012) Aryl hydrocarbon receptor-mediated impairment of chondrogenesis and fracture healing by cigarette smoke and benzo(a)pyrene. J Cell Physiol 227:1062–1070
Hanifi A, Richardson JB, Kuiper JH et al (2012) Clinical outcome of autologous chondrocyte implantation is correlated with infrared spectroscopic imaging-derived parameters. Osteoarthritis Cartilage 20:988–996
Halonen KS, Mononen ME, Jurvelin JS et al (2013) Importance of depth-wise distribution of collagen and proteoglycans in articular cartilage–a 3D finite element study of stresses and strains in human knee joint. J Biomech 46:1184–1192
Vaughan-Thomas A, Dudhia J, Bayliss MT et al (2008) Modification of the composition of articular cartilage collagen fibrils with increasing age. Connect Tissue Res 49:374–382
Gerstenfeld LC, Landis WJ (1991) Gene expression and extracellular matrix ultrastructure of a mineralizing chondrocyte cell culture system. J Cell Biol 112:501–513
Leboy PS, Vaias L, Uschmann B et al (1989) Ascorbic acid induces alkaline phosphatase, type X collagen, and calcium deposition in cultured chick chondrocytes. J Biol Chem 264:17281–17286
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Jonason, J.H., Hoak, D., O’Keefe, R.J. (2015). Primary Murine Growth Plate and Articular Chondrocyte Isolation and Cell Culture. In: Westendorf, J., van Wijnen, A. (eds) Osteoporosis and Osteoarthritis. Methods in Molecular Biology, vol 1226. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1619-1_2
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DOI: https://doi.org/10.1007/978-1-4939-1619-1_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1618-4
Online ISBN: 978-1-4939-1619-1
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