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Growth of Human Rhinovirus in H1-HeLa Cell Suspension Culture and Purification of Virions

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Book cover Rhinoviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1221))

Abstract

HeLa cell culture is the most widely used system for in vitro studies of the basic biology of human rhinovirus (HRV). It is also useful for making sufficient quantities of virus for experiments that require highly concentrated and purified virus. This chapter describes the protocols for producing a large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and making highly concentrated virus stock and highly purified virions. These purified HRV virions are free of cellular components and suitable for experiments that are sensitive to cellular contaminations.

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Author information

Authors and Affiliations

  1. Biological Mimetics Inc., 124 Byte Drive, Frederick, MD, 21702, USA

    Wai-Ming Lee

  2. Department of Pharmacology and Toxicology, School of Pharmacy, University of Arizona, Tucson, AZ, USA

    Yin Chen

  3. Global Biological Development, Bayer Healthcare, Berkeley, CA, USA

    Wensheng Wang

  4. Department of Medicine, University of Wisconsin, Madison, WI, USA

    Anne Mosser

Authors
  1. Wai-Ming Lee
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  2. Yin Chen
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  3. Wensheng Wang
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  4. Anne Mosser
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Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Faculty of Health, Nursing and Medical Sciences,, Monash University, Melbourne, Victoria, Australia

    David A. Jans

  2. Centre for Research in Therapeutic Solutions, Faculty of Education, Science, Technology and Mathematics, University of Canberra, Canberra, Aust Capital Terr, Australia

    Reena Ghildyal

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Lee, WM., Chen, Y., Wang, W., Mosser, A. (2015). Growth of Human Rhinovirus in H1-HeLa Cell Suspension Culture and Purification of Virions. In: Jans, D., Ghildyal, R. (eds) Rhinoviruses. Methods in Molecular Biology, vol 1221. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1571-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-1571-2_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1570-5

  • Online ISBN: 978-1-4939-1571-2

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