Skip to main content
SpringerLink
Log in
Book cover

Mast Cells pp 461–486Cite as

Studying Mast Cells in Peripheral Tolerance by Using a Skin Transplantation Model

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1220))

Abstract

Mast cells (MCs) play an important role in both inflammatory and immunosuppressive responses [1]. The importance of MCs in maintaining peripheral tolerance was discovered in a FoxP3+ regulatory T-cell (Treg)-mediated skin transplant model [2]. MCs can directly mediate tolerance by releasing anti-inflammatory mediators (reviewed in ref. 3) or by interacting with other immune cells in the graft. Here we will present protocols used to study the role of MCs in peripheral tolerance with the emphasis on how MCs can regulate T-cell functionality. First we will introduce the skin transplant model followed by reconstitution of mast cell-deficient mice (B6.Cg-Kit W-sh). This includes the preparation of MCs from the bone marrow. Finally the methods used to study the influence of MCs on T-cell responses and Treg functionality will be presented by modulating the balance between tolerance and inflammation.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Galli SJ, Grimbaldeston M, Tsai M (2008) Immunomodulatory mast cells: negative, as well as positive, regulators of immunity. Nat Rev Immunol 8(6):478–486

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Lu LF et al (2006) Mast cells are essential intermediaries in regulatory T-cell tolerance. Nature 442(7106):997–1002

    Article  CAS  PubMed  Google Scholar 

  3. de Vries VC, Noelle RJ (2010) Mast cell mediators in tolerance. Curr Opin Immunol 22(5):643–648

    Article  PubMed  Google Scholar 

  4. de Vries VC et al (2011) Mast cells condition dendritic cells to mediate allograft tolerance. Immunity 35(4):550–561

    Article  PubMed Central  PubMed  Google Scholar 

  5. de Vries VC et al (2009) Mast cell degranulation breaks peripheral tolerance. Am J Transplant 9(10):2270–2280

    Article  PubMed  Google Scholar 

  6. de Vries VC et al (2009) The enigmatic role of mast cells in dominant tolerance. Curr Opin Organ Transplant 14(4):332–337

    Article  PubMed  Google Scholar 

  7. Miller DM et al (2009) TLR agonists prevent the establishment of allogeneic hematopoietic chimerism in mice treated with costimulation blockade. J Immunol 182(9):5547–5559

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Elgueta R et al (2009) Molecular mechanism and function of CD40/CD40L engagement in the immune system. Immunol Rev 229(1):152–172

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Allergy and Inflammation, Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore

    Victor C. de Vries

  2. Department of Microbiology and Immunology, Dartmouth Medical School and Norris Cotton Cancer Center, Lebanon, NH, 03756, USA

    Isabelle Le Mercier, Elizabeth C. Nowak & Randolph J. Noelle

  3. King’s Health Partners, Medical Research Council (MRC) Centre of Transplantation, King’s College London, Guy’s Hospital, 5th Floor Tower Wing, London, SE1 9RT, UK

    Randolph J. Noelle

  4. Norris Cotton Cancer Center, One Medical Center Drive, Rubin Bldg., Room 730, Lebanon, NH, 03755, USA

    Randolph J. Noelle

Authors
  1. Victor C. de Vries
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Isabelle Le Mercier
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Elizabeth C. Nowak
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Randolph J. Noelle
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Randolph J. Noelle .

Editor information

Editors and Affiliations

  1. Department of Medical Genetics, The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada

    Michael R. Hughes

  2. Department of Medical Genetics, The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada

    Kelly M. McNagny

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

de Vries, V.C., Le Mercier, I., Nowak, E.C., Noelle, R.J. (2015). Studying Mast Cells in Peripheral Tolerance by Using a Skin Transplantation Model. In: Hughes, M., McNagny, K. (eds) Mast Cells. Methods in Molecular Biology, vol 1220. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1568-2_28

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-1568-2_28

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1567-5

  • Online ISBN: 978-1-4939-1568-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation