Abstract
Over the past decades, research on in vivo functions of mast cells has largely relied on kit-mutant mouse strains. Recently, new mouse models for investigation of mast cell functions based on the Cre/loxP recombination system have been published and results in these new models challenged findings of previous studies in kit-mutant mice. Herein we describe procedures central to mast cell-specific gene inactivation and the generation of mast cell-deficient mice based on the mouse strain Mcpt5-Cre, which expresses Cre recombinase selectively in connective tissue mast cells.
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Acknowledgments
This work was supported by research grants from the German Research Council to A.D. (DFG, Du1172/1; Du1172/2, Priority Program 1468), K.H. (DFG, CRC/SFB832, project A14; Ro2133/2), and A.R. (DFG, Ro2133/2; Ro2133/3; Ro2133/4, Priority Program 1394) and from the German-Israeli Foundation for Scientific Research and Development to K.H. (993/2008).
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Peschke, K., Dudeck, A., Rabenhorst, A., Hartmann, K., Roers, A. (2015). Cre/loxP-Based Mouse Models of Mast Cell Deficiency and Mast Cell-Specific Gene Inactivation. In: Hughes, M., McNagny, K. (eds) Mast Cells. Methods in Molecular Biology, vol 1220. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1568-2_25
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DOI: https://doi.org/10.1007/978-1-4939-1568-2_25
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