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Mast Cells pp 307–323Cite as

Assay of Mast Cell Mediators

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1220))

Abstract

Mediator release from activated mast cells is a major initiator of the symptomology associated with allergic disorders such as anaphylaxis and asthma. Thus, methods to monitor the generation and release of such mediators have widespread applicability in studies designed to understand the processes regulating mast cell activation and for the identification of therapeutic approaches to block mast cell-driven disease. In this chapter, we discuss approaches used for the determination of mast cell degranulation, lipid-derived inflammatory mediator production, and cytokine/chemokine gene expression as well as cytokine release.

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Abbreviations

BMMC:

Bone marrow-derived mast cells

FcεRI:

High-affinity IgE receptor

HuMC:

Human mast cell

IL:

Interleukin

LT:

Leukotriene

PG:

Prostaglandin

TNF-α:

Tumor necrosis factor-alpha

References

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Acknowledgments

Financial support for work in the authors’ laboratories was provided by the Division of Intramural Research of NIAID within the National Institutes of Health (AMG), VBG-GROUP Centre for Asthma and Allergy Research, Herman Krefting Foundation Against Asthma and Allergy (MR), Wellcome Trust Value in People Award, and Faculty of Medicine, University of Southampton (EJS).

Author information

Authors and Affiliations

  1. Krefting Research Centre, The Sahlgrenska Academy, University of Gothenburg, 424, Gothenburg, 405 30, Sweden

    Madeleine Rådinger Ph.D.

  2. Allergy Clinic, Copenhagen University Hospital, Gentofte, Denmark

    Bettina M. Jensen

  3. Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK

    Emily Swindle

  4. Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 10 Center Drive MSC 1881, Bethesda, MD, 20892-1881, USA

    Alasdair M. Gilfillan

Authors
  1. Madeleine Rådinger Ph.D.
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  2. Bettina M. Jensen
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  3. Emily Swindle
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  4. Alasdair M. Gilfillan
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Corresponding author

Correspondence to Madeleine Rådinger Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Medical Genetics, The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada

    Michael R. Hughes

  2. Department of Medical Genetics, The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada

    Kelly M. McNagny

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© 2015 Springer Science+Business Media New York

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Rådinger, M., Jensen, B.M., Swindle, E., Gilfillan, A.M. (2015). Assay of Mast Cell Mediators. In: Hughes, M., McNagny, K. (eds) Mast Cells. Methods in Molecular Biology, vol 1220. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1568-2_19

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  • DOI: https://doi.org/10.1007/978-1-4939-1568-2_19

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1567-5

  • Online ISBN: 978-1-4939-1568-2

  • eBook Packages: Springer Protocols

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