Skip to main content
SpringerLink
Log in
Book cover

Vascular Morphogenesis pp 173–184Cite as

Zebrafish Embryo Intersegmental Vessels: A Tool for Investigating Sprouting Angiogenesis

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1214))

Abstract

Angiogenesis leads to the formation of the intersegmental vessels (ISVs) of the trunk in teleost zebrafish (Danio rerio) embryo. Here we describe experimental procedures, including in vivo observation of transgenic zebrafish embryo and whole mount in situ hybridization, to investigate ISV development in zebrafish embryos and assess the effect of antiangiogenic compounds on these vessels.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Lam SH, Wu YL, Vega VB, Miller LD, Spitsbergen J, Tong Y et al (2006) Conservation of gene expression signatures between zebrafish and human liver tumors and tumor progression. Nat Biotechnol 24:73–75

    Article  CAS  PubMed  Google Scholar 

  2. Pichler FB, Laurenson S, Williams LC, Dodd A, Copp BR, Love DR et al (2003) Chemical discovery and global gene expression analysis in zebrafish. Nat Biotechnol 21:879–883

    Article  CAS  PubMed  Google Scholar 

  3. Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA (2008) Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol 26:695–701

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Funfak A, Brosing A, Brand M, Köhler JM (2007) Micro fluid segment technique for screening and development studies on Danio rerio embryos. Lab Chip 7:1132–1138

    Article  CAS  PubMed  Google Scholar 

  5. Weinstein B (2002) Vascular cell biology in vivo: a new piscine paradigm? Trends Cell Biol 12:439–445

    Article  CAS  PubMed  Google Scholar 

  6. Isogai S, Horiguchi M, Weinstein BM (2001) The vascular anatomy of the developing zebrafish: an atlas of embryonic and early larval development. Dev Biol 230:278–301

    Article  CAS  PubMed  Google Scholar 

  7. Baldessari D, Mione M (2008) How to create the vascular tree? (Latest) help from the zebrafish. Pharmacol Ther 118:206–230

    Article  CAS  PubMed  Google Scholar 

  8. Thisse C, Thisse B (2008) High-resolution in situ hybridization to whole-mount zebrafish embryos. Nat Protoc 3:59–69

    Article  CAS  PubMed  Google Scholar 

  9. Serbedzija GN, Flynn E, Willett CE (1999) Zebrafish angiogenesis: a new model for drug screening. Angiogenesis 3:353–359

    Article  CAS  PubMed  Google Scholar 

  10. Yaniv K, Isogai S, Castranova D, Dye L, Hitomi J, Weinstein BM (2006) Live imaging of lymphatic development in the zebrafish. Nat Med 12:711–716

    Article  CAS  PubMed  Google Scholar 

  11. Ellertsdottir E, Lenard A, Blum Y, Krudewig A, Herwig L, Affolter M et al (2010) Vascular morphogenesis in the zebrafish embryo. Dev Biol 341:56–65

    Article  CAS  PubMed  Google Scholar 

  12. Blum Y, Belting HG, Ellertsdottir E, Herwig L, Lüders F, Affolter M (2008) Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo. Dev Biol 316:312–322

    Article  CAS  PubMed  Google Scholar 

  13. Gerhardt H, Golding M, Fruttiger M, Ruhrberg C, Lundkvist A, Abramsson A et al (2003) VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. J Cell Biol 161:1163–1177

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. Herbert SP, Cheung JY, Stainier DY (2012) Determination of endothelial stalk versus tip cell potential during angiogenesis by H2.0-like homeobox-1. Curr Biol 22:1789–1794

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  15. Hasan J, Shnyder SD, Bibby M, Double JA, Bicknel R, Jayson GC (2004) Quantitative angiogenesis assays in vivo–a review. Angiogenesis 7:1–16

    Article  CAS  PubMed  Google Scholar 

  16. Belleri M, Ribatti D, Nicoli S, Cotelli F, Forti L, Vannini V et al (2005) Antiangiogenic and vascular-targeting activity of the microtubule-destabilizing trans-resveratrol derivative 3,5,4'-trimethoxystilbene. Mol Pharmacol 67:1451–1459

    Article  CAS  PubMed  Google Scholar 

  17. Yeh JC, Cindrova-Davies T, Belleri M, Morbidelli L, Miller N, Cho CW et al (2011) The natural compound n-butylidenephthalide derived from the volatile oil of Radix Angelica sinensis inhibits angiogenesis in vitro and in vivo. Angiogenesis 14:187–197

    Article  CAS  PubMed  Google Scholar 

  18. Nicoli S, Ribatti D, Cotelli F, Presta M (2007) Mammalian tumor xenografts induce neovascularization in zebrafish embryos. Cancer Res 67:2927–2931

    Article  CAS  PubMed  Google Scholar 

  19. Quaife NM, Watson O, Chico TJ (2012) Zebrafish: an emerging model of vascular development and remodelling. Curr Opin Pharmacol 12:608–614

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported in part by grants from Ministerodell’ Istruzione, Università e Ricerca (MIUR, Centro IDET, FIRB project RBAP11H2R9 2011) and Associazione Italiana per la Ricerca sul Cancro (AIRC grant no. 10396) to MP.

Author information

Authors and Affiliations

  1. Department of Molecular and Translational Medicine, Experimental Oncology and Immunology, School of Medicine, University of Brescia, Brescia, Italy

    Chiara Tobia, Giuseppina Gariano, Jessica Guerra & Marco Presta

Authors
  1. Chiara Tobia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Giuseppina Gariano
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jessica Guerra
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marco Presta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marco Presta .

Editor information

Editors and Affiliations

  1. University of Bari Aldo Moro, Bari, Italy

    Domenico Ribatti

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

Tobia, C., Gariano, G., Guerra, J., Presta, M. (2015). Zebrafish Embryo Intersegmental Vessels: A Tool for Investigating Sprouting Angiogenesis. In: Ribatti, D. (eds) Vascular Morphogenesis. Methods in Molecular Biology, vol 1214. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1462-3_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-1462-3_10

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1461-6

  • Online ISBN: 978-1-4939-1462-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation