MicroRNA In Situ Hybridization in Tissue Microarrays

  • Julia J. Turnock-Jones
  • John P. C. Le QuesneEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1211)


In situ hybridization is used to visualize nucleic acids in microscopic tissue sections and has in recent years been used successfully to detect microRNAs. We have further optimized a technique to detect and semiquantitatively assay microRNA expression in tissue microarrays derived from formalin-fixed paraffin-embedded archival tumor tissue.

Key words

In situ hybridization Tissue microarray FFPE Locked nucleic acid (LNA) microRNA 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Julia J. Turnock-Jones
    • 1
  • John P. C. Le Quesne
    • 2
    Email author
  1. 1.Cancer Research UK, Cambridge Institute for Julia Turnock-JonesUniversity of CambridgeCambridgeUK
  2. 2.Cancer Research UK, Cambridge Research InstituteUniversity Hospitals of Leicester NHS Trust, University of LeicesterLeicesterUK

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