MicroRNA In Situ Hybridization in Tissue Microarrays
In situ hybridization is used to visualize nucleic acids in microscopic tissue sections and has in recent years been used successfully to detect microRNAs. We have further optimized a technique to detect and semiquantitatively assay microRNA expression in tissue microarrays derived from formalin-fixed paraffin-embedded archival tumor tissue.
Key wordsIn situ hybridization Tissue microarray FFPE Locked nucleic acid (LNA) microRNA
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