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Whole-Mount In Situ Hybridization Using DIG-Labeled Probes in Planarian

  • Agnieszka Rybak-WolfEmail author
  • Jordi Solana
Part of the Methods in Molecular Biology book series (MIMB, volume 1211)

Abstract

In recent years freshwater flatworms (planarian) have become a powerful model for studies of regeneration and stem cell biology. Whole-mount in situ hybridization (WISH) and fluorescent in situ hybridization (FISH) are key and most commonly used techniques to determine and visualize gene expression patterns in planaria. Here, we present the established version of whole-mount in situ hybridization (WISH) and whole-mount fluorescence in situ hybridization (WFISH) protocol optimized over the last years by several labs from the rapidly growing planaria field and give an overview of recently introduced modifications which can be critical in the study of low abundant transcripts.

Key words

Planarian Schmidtea mediterranea Whole-mount in situ hybridization (WISH) Fluorescent in situ hybridization (FISH) DIG-labeled probes 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Laboratory for Systems Biology of Gene Regulatory ElementsBerlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular MedicineBerlinGermany

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