Identification of Low-Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia
Detection of low-expressing transcripts in tissues is a major technical challenge that requires considerate advance planning. To produce high-quality publishable data, many controls need to be employed, including knock out animal models, independent assays, and high-end imaging techniques. The current protocol describes the use of commercial kit, QuantiGene ViewRNA 1-plex assay for a reliable detection of low-expressing transcripts in formalin-fixed paraffin-embedded murine tissues. Examples of positive (high-expressing) and negative (knock out) control samples are provided to describe a case study.
Key wordsIn situ hybridization ISH GPCR NPY receptor/s Basal lingual epithelia Taste receptor cells