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Identification of Low-Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia

  • Sergei ZolotukhinEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1211)

Abstract

Detection of low-expressing transcripts in tissues is a major technical challenge that requires considerate advance planning. To produce high-quality publishable data, many controls need to be employed, including knock out animal models, independent assays, and high-end imaging techniques. The current protocol describes the use of commercial kit, QuantiGene ViewRNA 1-plex assay for a reliable detection of low-expressing transcripts in formalin-fixed paraffin-embedded murine tissues. Examples of positive (high-expressing) and negative (knock out) control samples are provided to describe a case study.

Key words

In situ hybridization ISH GPCR NPY receptor/s Basal lingual epithelia Taste receptor cells 

References

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    Watakabe A, Komatsu Y et al (2010) Fluorescent in situ hybridization technique for cell type identification and characterization in the central nervous system. Methods 52:367–374PubMedCrossRefGoogle Scholar
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    Wang F, Flanagan J et al (2012) RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn 14:22–29PubMedCrossRefPubMedCentralGoogle Scholar
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    Hurtado MD, Acosta A et al (2012) Distribution of Y-receptors in murine lingual epithelia. PLoS One 7:e46358PubMedCrossRefPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Division of Cell and Molecular Therapy, Cancer and Genetics Research ComplexUniversity of FloridaGainesvilleUSA

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