Identification of Low-Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia

  • Sergei ZolotukhinEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1211)


Detection of low-expressing transcripts in tissues is a major technical challenge that requires considerate advance planning. To produce high-quality publishable data, many controls need to be employed, including knock out animal models, independent assays, and high-end imaging techniques. The current protocol describes the use of commercial kit, QuantiGene ViewRNA 1-plex assay for a reliable detection of low-expressing transcripts in formalin-fixed paraffin-embedded murine tissues. Examples of positive (high-expressing) and negative (knock out) control samples are provided to describe a case study.

Key words

In situ hybridization ISH GPCR NPY receptor/s Basal lingual epithelia Taste receptor cells 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Division of Cell and Molecular Therapy, Cancer and Genetics Research ComplexUniversity of FloridaGainesvilleUSA

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