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Bone Marrow Stromal Stem Cells Transplantation in Mice with Acute Spinal Cord Injury

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Animal Models for Stem Cell Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1213))

Abstract

Spinal cord injured experimental animals are widely used for studying pathophysiological processes after central nervous system acute traumatic lesion and elaborating therapeutic solutions, some of them based on stem cell transplantation. Here, we describe a protocol of spinal cord contusion in C57BL/6J mice, directly followed by bone marrow stromal stem cells transplantation. This model allows for the characterization of neuroprotective and neurorestorative abilities of these stem cells in a context of spinal cord trauma.

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Acknowledgements

This work was supported by a grant from the Fonds National de la Recherche Scientifique (FNRS) of Belgium, by the Belgian League against Multiple Sclerosis associated with the Leon Frédericq Foundation, and by the Fonds Spéciaux à la Recherche of the University of Liège.

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Authors and Affiliations

  1. Groupe Interdisciplinaire de Génoprotéomique appliquée (GIGA), Neurosciences Unit, University of Liège, Avenue de l’Hopital 1 – B36, Liège, 4000, Belgium

    Virginie Neirinckx, Bernard Rogister, Rachelle Franzen & Sabine Wislet-Gendebien

  2. Groupe Interdisciplinaire de Génoprotéomique appliquée (GIGA), Development, Stem Cells and Regenerative Medicine Unit, University of Liège, Liège, Belgium

    Bernard Rogister

  3. Neurology Department, Centre Hospitalier Universitaire de Liège, Liège, Belgium

    Bernard Rogister

Authors
  1. Virginie Neirinckx
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  2. Bernard Rogister
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  3. Rachelle Franzen
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  4. Sabine Wislet-Gendebien
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Corresponding author

Correspondence to Sabine Wislet-Gendebien .

Editor information

Editors and Affiliations

  1. Department of Surgery, Unviersity of Leipzig, Germany

    Bruno Christ

  2. Department of Surgery, University of Leipzig, Germany

    Jana Oerlecke

  3. Department of Surgery, University of Leipzig, Germany

    Peggy Stock

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Neirinckx, V., Rogister, B., Franzen, R., Wislet-Gendebien, S. (2014). Bone Marrow Stromal Stem Cells Transplantation in Mice with Acute Spinal Cord Injury. In: Christ, B., Oerlecke, J., Stock, P. (eds) Animal Models for Stem Cell Therapy. Methods in Molecular Biology, vol 1213. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1453-1_21

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  • DOI: https://doi.org/10.1007/978-1-4939-1453-1_21

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1452-4

  • Online ISBN: 978-1-4939-1453-1

  • eBook Packages: Springer Protocols

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