Abstract
Mesenchymal stem cells (MSCs) can be obtained from adult bone marrow and adipose tissue in large quantities and are the main cell types that contribute to recovery from ischemia because, among their biological activities, they produce several proangiogenic paracrine factors and differentiate into endothelial cells. Mouse hind limb ischemia induced by surgery is a useful animal model to study the angiogenic properties of MSCs, but it requires several precautions to be reproducible. The preparation of MSCs, the ischemic surgery, and the physiological and histological analyses are described in detail.
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Acknowledgments
L.M. and P.K.M.M. are recipients of FAPESP scholarships. This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2011/00859-6 and 2012/21861-1).
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Martins, L., Martin, P.K.M., Han, S.W. (2014). Angiogenic Properties of Mesenchymal Stem Cells in a Mouse Model of Limb Ischemia. In: Christ, B., Oerlecke, J., Stock, P. (eds) Animal Models for Stem Cell Therapy. Methods in Molecular Biology, vol 1213. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1453-1_13
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DOI: https://doi.org/10.1007/978-1-4939-1453-1_13
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