Abstract
A comparison of crude curcuminoid extract and purified curcumin was made to evaluate the immunoprotective effect of Curcuma longa (turmeric) Zingiberaceae. Carbon tetrachloride (CCl4) induced selective cytolytic effects among immature (PNA+) thymocytes and peripheral helper (CD4+) T lymphocytes in the spleen were paralleled by a significant reduction in CD25, CD71, and Con A receptor expression. Treatment with curcumanoid crude extract, at two different doses, showed a significant restoration of lymphocyte viability and CD25, CD71, and Con A receptor expression in both immature (PNA+) thymocytes and splenic helper (CD4+) T lymphocytes. Turmeric crude extract, at both low and high dose, was found to be more efficient as compared to purified curcumin, suggesting synergistic effect of curcumin with other components of the crude extract.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Mesel-Lemoine M, Cherai M, Le Gouvello S, Guillot M, Leclercq V, Klatzmann D, Thomas-Vaslin V, Lemoine FM (2005) Immune dysfunctions of cultured T lymphocytes are due to a preferential expansion of CD4+CD25+ suppressor cells. Blood 106:5199
Rukkumani R, Aruna K, Varma PS, Rajasekaran KN, Menon VP (2004) Comparative effects of curcumin and an analog of curcumin on alcohol and PUFA induced oxidative stress. J Pharm Pharm Sci 7:274–283
Fujisawa S, Atsumi T, Murakami Y, Kadoma Y (2005) Dimerization, ROS formation, and biological activity of o-methoxyphenols. Arch Immunol Ther Exp (Warsz) 53:28–38
Singh S, Khar A (2006) Biological effects of curcumin and its role in cancer chemoprevention and therapy. Anticancer Agents Med Chem 6:259–270
Tomas-Zapico C, Caballero B, Sierra V, Vega-Naredo I, Alvarez-Garcia O, Tolivia D, Rodriguez-Colunga MJ, Coto-Montes A (2005) Survival mechanisms in a physiological oxidative stress model. FASEB J 19:3594–3595
Haufe D, Luther T, Kotzsch M, Knels L, Koch T (2004) Perfluorocarbon attenuates response of concanavalin A-stimulated mononuclear blood cells without altering ligand-receptor interaction. Am J Physiol Lung Cell Mol Physiol 287:L/210–L/216
Chakravarty AK, Yasmin H (2005) Alcoholic turmeric extract simultaneously activating murine lymphocytes and inducing apoptosis of Ehlrich ascitic carcinoma cells. Int Immunopharmacol 5:1574–1581
Gautam SC, Gao X, Dulchavsky S (2007) Immunomodulation by curcumin. Adv Exp Med Biol 595:321–341
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Abu-Rizq, H.A., Mansour, M.H., Afzal, M. (2015). Curcuma longa Attenuates Carbon Tetrachloride-Induced Oxidative Stress in T-Lymphocyte Subpopulations. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress III. Methods in Molecular Biology, vol 1208. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1441-8_12
Download citation
DOI: https://doi.org/10.1007/978-1-4939-1441-8_12
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1440-1
Online ISBN: 978-1-4939-1441-8
eBook Packages: Springer Protocols