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Generation of Murine Xenograft Models of Brain Tumors from Primary Human Tissue for In Vivo Analysis of the Brain Tumor-Initiating Cell

  • Maleeha Qazi
  • Aneet Mann
  • Randy van Ommeren
  • Chitra Venugopal
  • Nicole McFarlane
  • Parvez Vora
  • Sheila K. Singh
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1210)

Abstract

The generation of xenograft models, which support the growth of human tissue in animals, forms an important part of a researcher’s tool kit and enhances the ability to understand the initiation and development of cancer in vivo. Especially in the context of the brain tumor-initiating cell (BTIC), a xenograft model allows for careful characterization of BTIC roles in tumor initiation, growth, and relapse. Here, we detail a set of procedures which describe the isolation, enrichment, and intracranial injection of human BTICs from patient samples to generate xenograft models of a human brain tumor.

Key words

Flow cytometry Brain tumor-initiating cell (BTIC) Neural stem cell Intracranial injection NOD SCIDs Xenograft 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Maleeha Qazi
    • 1
  • Aneet Mann
    • 1
  • Randy van Ommeren
    • 1
  • Chitra Venugopal
    • 1
  • Nicole McFarlane
    • 1
  • Parvez Vora
    • 1
  • Sheila K. Singh
    • 1
  1. 1.McMaster Children’s HospitalMcMaster UniversityHamiltonCanada

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