Protocol for Cutaneous Wound Healing Assay in a Murine Model
Cutaneous wound healing assay is important to address many key questions including (1) migration ability of different cells; (2) communication between the different cell types such as keratinocytes, fibroblasts, and immune cells; (3) understanding the cell-autonomous and non-cell-autonomous function(s) of the different cells; and (4) gene regulation in healing processes. Wound healing studies can be used to test new treatment modalities, function of new drugs/compounds, and stem cell-based therapies on the different stages of healing and for accelerating wound healing in patients with compromised healing. In this chapter, we have described a simple step-by-step protocol to generate full-thickness cutaneous wounds in the dorsal skin of mice, followed by collecting the post-wounding biopsied materials on specific days for histological and immunohistochemical analyses and for RNA and protein extractions.
Key wordsCutaneous wound healing Therapeutic targets Transcription factors Histology Immunohistochemistry Protein Biopsy Mice, Skin
I would like to thank Xiaobo Liang and Shreya Bhattacharya for the images. I would like to also thank Arup K. Indra for critical reading of the review article. This work was supported by NIH grant 5R01AR056008-02.