Abstract
RAB GTPases interact with specific effector molecules in a spatiotemporally regulated manner to induce various downstream reactions. To clarify the overall picture of RAB GTPase functions, it is important to elucidate the cellular locale where RAB and its effectors interact. Here, we applied a bimolecular fluorescence complementation (BiFC) assay to analyze where RAB GTPase interacted with effectors in endosomal trafficking.
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Acknowledgement
This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and from JST, PRESTO.
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Ito, E., Ueda, T. (2014). Analysis of Rab GTPase–Effector Interactions by Bimolecular Fluorescence Complementation. In: Otegui, M. (eds) Plant Endosomes. Methods in Molecular Biology, vol 1209. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1420-3_7
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DOI: https://doi.org/10.1007/978-1-4939-1420-3_7
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