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Analysis of Global Ubiquitylation and Ubiquitin-Binding Domains Involved in Endosomal Trafficking

  • Kamila Kalinowska
  • Erika IsonoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1209)

Abstract

Ubiquitylation is a reversible posttranslational modification that regulates various cellular pathways. Ubiquitylation of a plasma membrane protein was shown to serve as a signal for endocytosis of plasma membrane proteins in yeast and mammals as well as in plants. As more and more plant plasma membrane proteins are reported to be regulated through their ubiquitylation status, methods to analyze ubiquitylation and ubiquitin binding would be useful for the characterization of proteins involved in endocytosis of ubiquitylated cargo proteins.

Key words

Ubiquitin K48 K63 Western blot Binding assay 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Plant Systems BiologyTechnische Universität MünchenFreisingGermany

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