Galectins pp 133-151 | Cite as

Methods for Assessing the Effects of Galectins on Leukocyte Trafficking

  • Beatrice R. Gittens
  • Rachael D. Wright
  • Dianne CooperEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1207)


Numerous protocols exist for investigating leukocyte recruitment both in vitro and in vivo. Here we describe three of these methods; an in vitro flow chamber assay, intravital microscopy, and zymosan-induced peritonitis, and give details as to how they can be used to study the actions of galectins on this crucial process.

Key words

Leukocyte Neutrophil Endothelium Galectin Transmigration 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Beatrice R. Gittens
    • 1
  • Rachael D. Wright
    • 1
  • Dianne Cooper
    • 1
    Email author
  1. 1.William Harvey Research Institute, Barts and The London School of Medicine and DentistryQueen Mary University of LondonLondonUK

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