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Examining Galectin Binding Specificity Using Glycan Microarrays

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Galectins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1207))

Abstract

Glycan binding proteins (GBPs) possess the unique ability to regulate a wide variety of biological processes through interactions with highly modifiable cell surface glycans. While many studies demonstrate the impact of glycan modification on GBP recognition and activity, the relative contribution of subtle changes in glycan structure on GBP binding can be difficult to define. To overcome limitations in the analysis of GBP-glycan interactions, recent studies utilized glycan microarray platforms containing hundreds of structurally defined glycans. These studies not only provided important information regarding GBP–glycan interactions, but have also resulted in significant insight into the binding specificity and biological activity of the galectin family. We will describe the methods used when employing glycan microarray platforms to examine galectin–glycan binding specificity and function.

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Acknowledgments

This work was supported in part by grants from the National Blood Foundation, American Society of Hematology and Hemophilia of Georgia to S.R.S.

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Authors and Affiliations

  1. The Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA

    Connie M. Arthur & Harold C. Sullivan

  2. Faculty of Pharmaceutical Sciences of Ribeirão Preto, Department of Clinical, Toxicological and Bromatological Analysis, University of Sao Paulo, Ribeirão Preto-S, Brazil

    Lílian Cataldi Rodrigues & Marcelo Dias Baruffi

  3. Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA

    Jamie Heimburg-Molinaro, Dave F. Smith & Richard D. Cummings

  4. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 101 Woodruff Circle, 7307 WMB, Atlanta, GA, 30322, USA

    Sean R. Stowell

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  1. Connie M. Arthur
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  2. Lílian Cataldi Rodrigues
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  3. Marcelo Dias Baruffi
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  4. Harold C. Sullivan
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  5. Jamie Heimburg-Molinaro
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  6. Dave F. Smith
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  7. Richard D. Cummings
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  8. Sean R. Stowell
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Corresponding author

Correspondence to Sean R. Stowell .

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Editors and Affiliations

  1. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA

    Sean R. Stowell

  2. Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, Georgia, USA

    Richard D. Cummings

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Arthur, C.M. et al. (2015). Examining Galectin Binding Specificity Using Glycan Microarrays. In: Stowell, S., Cummings, R. (eds) Galectins. Methods in Molecular Biology, vol 1207. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1396-1_8

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  • DOI: https://doi.org/10.1007/978-1-4939-1396-1_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1395-4

  • Online ISBN: 978-1-4939-1396-1

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