Galectins pp 115-131 | Cite as

Examining Galectin Binding Specificity Using Glycan Microarrays

  • Connie M. Arthur
  • Lílian Cataldi Rodrigues
  • Marcelo Dias Baruffi
  • Harold C. Sullivan
  • Jamie Heimburg-Molinaro
  • Dave F. Smith
  • Richard D. Cummings
  • Sean R. StowellEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1207)


Glycan binding proteins (GBPs) possess the unique ability to regulate a wide variety of biological processes through interactions with highly modifiable cell surface glycans. While many studies demonstrate the impact of glycan modification on GBP recognition and activity, the relative contribution of subtle changes in glycan structure on GBP binding can be difficult to define. To overcome limitations in the analysis of GBP-glycan interactions, recent studies utilized glycan microarray platforms containing hundreds of structurally defined glycans. These studies not only provided important information regarding GBP–glycan interactions, but have also resulted in significant insight into the binding specificity and biological activity of the galectin family. We will describe the methods used when employing glycan microarray platforms to examine galectin–glycan binding specificity and function.

Key words

Glycan binding protein (GBP) Galectin Glycan microarray GBP–glycan interactions 



This work was supported in part by grants from the National Blood Foundation, American Society of Hematology and Hemophilia of Georgia to S.R.S.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Connie M. Arthur
    • 1
  • Lílian Cataldi Rodrigues
    • 2
  • Marcelo Dias Baruffi
    • 2
  • Harold C. Sullivan
    • 1
  • Jamie Heimburg-Molinaro
    • 3
  • Dave F. Smith
    • 3
  • Richard D. Cummings
    • 3
  • Sean R. Stowell
    • 4
    Email author
  1. 1.The Department of Pathology and Laboratory MedicineEmory University School of MedicineAtlantaUSA
  2. 2.Faculty of Pharmaceutical Sciences of Ribeirão Preto, Department of Clinical, Toxicological and Bromatological AnalysisUniversity of Sao PauloRibeirão Preto-SBrazil
  3. 3.Department of BiochemistryEmory University School of MedicineAtlantaUSA
  4. 4.Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory MedicineEmory University School of MedicineAtlantaUSA

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