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Galectins pp 105-114 | Cite as

Evaluation of Galectin Binding by Surface Plasmon Resonance

  • Padmaja Mehta-D’souzaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1207)

Abstract

Surface Plasmon Resonance (SPR) instruments, like the BIAcore 3000, are useful for studying the binding between macromolecules in real time. The high sensitivity and low sample consumption in the BIAcore enables the measurement of rapid kinetics and low affinities characteristic of many biological interactions. This chapter describes the affinity measurement of Galectins-1, -2, and -3 and their glycoside ligands using this approach.

Key words

Surface plasmon resonance (SPR) Streptavidin (SA) sensor chip Affinity Ligand Analyte Flow cell (fc) Galectin Glycosides 

Notes

Acknowledgments

The author thanks Drs. Sean R. Stowell, Richard D. Cummings, and Richard Alvarez for helpful discussions during the course of this study. The BIAcore 3000 instrument used in this study was purchased with an NIH shared instrumentation grant awarded to Rodger P. McEver. The study was carried out in the Biacore Core Facility at the Oklahoma Medical Research Foundation.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Oklahoma Medical Research CenterOklahoma CityUSA

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