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Galectins pp 371-395 | Cite as

Evaluation of the Role of Galectins in Parasite Immunity

  • Sarah Preston
  • Jillian Dunphy
  • Travis Beddoe
  • Els MeeusenEmail author
  • Anna Young
Part of the Methods in Molecular Biology book series (MIMB, volume 1207)

Abstract

Galectin-11 and galectin-14 are ruminant galectins involved in parasitic infections. Although their roles in parasite immunity are still being elucidated, its appears that their functions are parasite specific. In gastrointestinal infections with the nematode Haemonchus contortus, both galectin-11 and galectin-14 appear to be protective. However, in a chronic infection of liver fluke, Fasciola hepatica, these galectins may aid parasite survival. This chapter discusses the methods designed to study parasitic infections in sheep, which have provided us with insight into the functions of galectin-11 and galectin-14 during host–parasite interactions. These methods include parasite cultivation and infection, galectin staining of host and parasite tissue, surface staining of parasites with recombinant galectins and in vitro assays to monitor the effect of galectins on larval development.

Key words

Galectin-11 Galectin-14 Haemonchus contortus Fasciola hepatica Larval culture Immunohistochemistry Larval molting assay Larval growth assay Larval feeding assay 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Sarah Preston
    • 1
    • 2
  • Jillian Dunphy
    • 3
  • Travis Beddoe
    • 4
  • Els Meeusen
    • 5
    Email author
  • Anna Young
    • 6
  1. 1.Biotechnology Research Laboratories, School of Biomedical SciencesMonash UniversityClaytonAustralia
  2. 2.Cooperative Research Centre for Sheep Industry Innovation, CJ Hawkins HomesteadUniversity of New EnglandArmidaleAustralia
  3. 3.School of Community HealthCharles Sturt UniversityAlburyAustralia
  4. 4.Department of Agricultural Sciences Centre for AgriBiosciencesLa Trobe UniversityBundooraAustralia
  5. 5.Department of Microbiology, School of Biomedical SciencesMonash UniversityClaytonAustralia
  6. 6.Department of Physiology, School of Biomedical SciencesMonash UniversityClaytonAustralia

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