Evaluation of the Role of Galectins in Parasite Immunity
Galectin-11 and galectin-14 are ruminant galectins involved in parasitic infections. Although their roles in parasite immunity are still being elucidated, its appears that their functions are parasite specific. In gastrointestinal infections with the nematode Haemonchus contortus, both galectin-11 and galectin-14 appear to be protective. However, in a chronic infection of liver fluke, Fasciola hepatica, these galectins may aid parasite survival. This chapter discusses the methods designed to study parasitic infections in sheep, which have provided us with insight into the functions of galectin-11 and galectin-14 during host–parasite interactions. These methods include parasite cultivation and infection, galectin staining of host and parasite tissue, surface staining of parasites with recombinant galectins and in vitro assays to monitor the effect of galectins on larval development.
Key wordsGalectin-11 Galectin-14 Haemonchus contortus Fasciola hepatica Larval culture Immunohistochemistry Larval molting assay Larval growth assay Larval feeding assay
- 3.Pelletier I, Hashidate T, Urashima T, Nishill N, Nakamura T, Futai M, Arata Y, Kasai KI, Hirashima M, Hirabayashi J, Sato S (2003) Specific recognition of Leishmania major poly-β-galactosyl epitopes by galectin-9: possible implication of galectin-9 in interaction between L. major and host cells. J Biol Chem 278(25):22223–22230. doi: 10.1074/jbc.M302693200 PubMedCrossRefGoogle Scholar
- 4.Alves CMOS, Silva DAO, Azzolini AECS, Marzocchi-Machado CM, Carvalho JV, Pajuaba ACAM, Lucisano-Valim YM, Chammas R, Liu FT, Roque-Barreira MC, Mineo JR (2010) Galectin-3 plays a modulatory role in the life span and activation of murine neutrophils during early Toxoplasma gondii infection. Immunobiology 215(6):475–485. doi: 10.1016/j.imbio.2009.08.001 PubMedCrossRefGoogle Scholar
- 16.Ackerman SJ, Liu L, Kwatia MA, Savage MP, Leonidas DD, Jawahar Swaminathan G, Ravi Acharya K (2002) Charcot-leyden crystal protein (galectin-10) is not a dual function galectin with lysophospholipase activity but binds a lysophospholipase inhibitor in a novel structural fashion. J Biol Chem 277(17):14859–14868. doi: 10.1074/jbc.M200221200 PubMedCrossRefGoogle Scholar
- 24.Athanasiadou S, Pemberton A, Jackson F, Inglis N, Miller HRP, Thévenod F, Mackellar A, Huntley JF (2008) Proteomic approach to identify candidate effector molecules during the in vitro immune exclusion of infective Teladorsagia circumcincta in the abomasum of sheep. Vet Res 39(6):58PubMedCrossRefGoogle Scholar
- 25.Gray CA, Adelson DL, Bazer FW, Burghardt RC, Meeusen ENT, Spencer TE (2004) Discovery and characterization of an epithelial-specific galectin in the endometrium that forms crystals in the trophectoderm. Proc Natl Acad Sci U S A 101(21):7982–7987. doi: 10.1073/pnas.0402669101 PubMedCrossRefPubMedCentralGoogle Scholar