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Galectins pp 317-326 | Cite as

Examination of the Role of Galectins in Cell Migration and Re-epithelialization of Wounds

  • Zhiyi Cao
  • Chandrassegar Saravanan
  • Wei-Sheng Chen
  • Noorjahan PanjwaniEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1207)

Abstract

Re-epithelialization is a crucial step for wound healing. As galectins play important roles in re-epithelialization, we describe here protocols for in vivo, ex vivo and in vitro examination of the role of galectins in cell migration and in re-epithelialization of wounds. For in vivo models, mouse corneas are wounded by a variety of techniques and the rate of re-epithelialization is quantified. For ex vivo organ culture models, mouse corneas are wounded in situ, the eyes are enucleated, the eyeballs are cultured in the presence or absence of galectins and the rate of re-epithelialization is quantified. For cell cultured-based in vitro assays, we examine formation of lamellipodia and activation of focal adhesion kinase in various epithelial cells.

Key words

Galectins Cell migration Re-epithelialization Wound healing Cornea Epithelial cells Lamellipodia Focal adhesion kinase 

Notes

Acknowledgement

The work carried out in the authors’ laboratory was supported by National Institutes of Health Grants EY009349 and EY007088; Massachusetts Lions Eye Research Fund, New England Corneal Transplant Fund and an unrestricted award from Research to Prevent Blindness.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Zhiyi Cao
    • 1
  • Chandrassegar Saravanan
    • 2
  • Wei-Sheng Chen
    • 2
  • Noorjahan Panjwani
    • 3
    Email author
  1. 1.New England Eye Center and Department of OphthalmologyTufts UniversityBostonUSA
  2. 2.Program in Cell, Molecular and Developmental Biology, Sackler School of Graduate Biomedical SciencesTufts UniversityBostonUSA
  3. 3.New England Eye Center and Departments of Ophthalmology and Developmental, Molecular and Chemical BiologyTufts UniversityBostonUSA

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