Examination of the Role of Galectins in Cell Migration and Re-epithelialization of Wounds
Re-epithelialization is a crucial step for wound healing. As galectins play important roles in re-epithelialization, we describe here protocols for in vivo, ex vivo and in vitro examination of the role of galectins in cell migration and in re-epithelialization of wounds. For in vivo models, mouse corneas are wounded by a variety of techniques and the rate of re-epithelialization is quantified. For ex vivo organ culture models, mouse corneas are wounded in situ, the eyes are enucleated, the eyeballs are cultured in the presence or absence of galectins and the rate of re-epithelialization is quantified. For cell cultured-based in vitro assays, we examine formation of lamellipodia and activation of focal adhesion kinase in various epithelial cells.
Key wordsGalectins Cell migration Re-epithelialization Wound healing Cornea Epithelial cells Lamellipodia Focal adhesion kinase
The work carried out in the authors’ laboratory was supported by National Institutes of Health Grants EY009349 and EY007088; Massachusetts Lions Eye Research Fund, New England Corneal Transplant Fund and an unrestricted award from Research to Prevent Blindness.
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