Galectins pp 305-315 | Cite as

Examination of the Role of Galectins During In Vivo Angiogenesis Using the Chick Chorioallantoic Membrane Assay

  • Esther A. Kleibeuker
  • Iris A. E. Schulkens
  • Kitty C. M. Castricum
  • Arjan W. Griffioen
  • Victor L. J. L. ThijssenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1207)


Angiogenesis is a complex multi-process involving various activities of endothelial cells. These activities are influenced in vivo by environmental conditions like interactions with other cell types and the microenvironment. Galectins play a role in several of these interactions and are therefore required for proper execution of in vivo angiogenesis. In this chapter we describe a method to study galectins and galectin inhibitors during physiologic and pathophysiologic angiogenesis in vivo using the chicken chorioallantoic membrane (CAM) assay.

Key words

Chorioallantoic membrane (CAM) assay Chicken Angiogenesis Galectin Tumor graft Blood vessel Vasculature 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Esther A. Kleibeuker
    • 1
  • Iris A. E. Schulkens
    • 2
  • Kitty C. M. Castricum
    • 3
  • Arjan W. Griffioen
    • 2
  • Victor L. J. L. Thijssen
    • 2
    • 3
    Email author
  1. 1.Angiogenesis Laboratory Amsterdam, Department of Radiation OncologyVU University medical centerAmsterdamThe Netherlands
  2. 2.Angiogenesis Laboratory Amsterdam, Department of Medical OncologyVU University medical centerAmsterdamThe Netherlands
  3. 3.Angiogenesis Laboratory Amsterdam, Department of Radiation OncologyVU University medical centerAmsterdamThe Netherlands

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