Galectins pp 285-291 | Cite as

Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology

  • Iris A. E. Schulkens
  • Esther A. Kleibeuker
  • Kitty C. M. Castricum
  • Arjan W. Griffioen
  • Victor L. J. L. ThijssenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1207)


The growth of new blood vessels is a key event in many (patho)physiological processes, including embryogenesis, wound healing, inflammatory diseases, and cancer. Neovascularization requires different, well-coordinated actions of endothelial cells, i.e., the cells lining the luminal side of all blood vessels. Galectins are involved in several of these activities. In this chapter we describe methods to study galectins and galectin inhibition in three key functions of endothelial cells during angiogenesis, i.e., endothelial cell migration, endothelial cell sprouting, and endothelial cell network formation.

Key words

Angiogenesis Endothelial cell Migration Network formation Sprouting Galectin 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Iris A. E. Schulkens
    • 1
  • Esther A. Kleibeuker
    • 2
  • Kitty C. M. Castricum
    • 2
  • Arjan W. Griffioen
    • 1
  • Victor L. J. L. Thijssen
    • 1
    • 2
    Email author
  1. 1.Angiogenesis Laboratory Amsterdam, Department of Medical OncologyVU University medical centerAmsterdamThe Netherlands
  2. 2.Angiogenesis Laboratory Amsterdam, Department of Radiation OncologyVU University medical centerAmsterdamThe Netherlands

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