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Galectins pp 249–268Cite as

Study of Galectins in Tumor Immunity: Strategies and Methods

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1207))

Abstract

During the past decade, a better understanding of the cellular and molecular mechanisms underlying tumor immunity has provided the appropriate framework for the development of therapeutic strategies for cancer immunotherapy. Under this complex scenario, galectins have emerged as promising molecular targets for cancer therapy responsible of creating immunosuppressive microenvironments at sites of tumor growth and metastasis. Galectins, expressed in tumor, stromal, and endothelial cells, contribute to thwart the development of immune responses by favoring the expansion of T regulatory cells and contributing to their immunosuppressive activity, driving the differentiation of tolerogenic dendritic cells, limiting T cell viability, and maintaining T cell anergy. The emerging data promise a future scenario in which the selective blockade of individual members of the galectin family, either alone or in combination with other therapeutic regimens, will contribute to halt tumor progression by counteracting tumor-immune escape. Here we describe a selection of methods used to investigate the role of galectin-1 in tumor-immune escape.

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Acknowledgements

Work in our laboratory is supported by grants from The Argentinean Agency for Promotion of Science and Technology (G.A.R, M.S., D.J.L, M.A.T, D.C.), University of Buenos Aires (G.A.R), Prostate Cancer Action (G.A.R., D.J.L, D.C), Argentinean Council of Scientific and Technical Investigations (M.S), National Multiple Sclerosis Society (G.A.R.), Broad Foundation (G.A.R.), and Sales Foundation (G.A.R).

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Authors and Affiliations

  1. Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1428, Buenos Aires, Argentina

    Juan P. Cerliani, Tomas Dalotto-Moreno, L. Sebastián Dergan-Dylon, Diego O. Croci, Santiago P. Méndez-Huergo, Marta A. Toscano & Mariana Salatino

  2. Laboratorio de Glicómica Funcional, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina

    Daniel Compagno, Diego J. Laderach & Lucas Gentilini

  3. Laboratorio de Glicómica Funcional, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales and Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental (IBYME), Universidad de Buenos Aires and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    Gabriel A. Rabinovich

Authors
  1. Juan P. Cerliani
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  2. Tomas Dalotto-Moreno
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  3. Daniel Compagno
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  4. L. Sebastián Dergan-Dylon
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  5. Diego J. Laderach
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  6. Lucas Gentilini
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  7. Diego O. Croci
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  8. Santiago P. Méndez-Huergo
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  9. Marta A. Toscano
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  10. Mariana Salatino
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  11. Gabriel A. Rabinovich
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Corresponding author

Correspondence to Juan P. Cerliani .

Editor information

Editors and Affiliations

  1. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA

    Sean R. Stowell

  2. Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, Georgia, USA

    Richard D. Cummings

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Cerliani, J.P. et al. (2015). Study of Galectins in Tumor Immunity: Strategies and Methods. In: Stowell, S., Cummings, R. (eds) Galectins. Methods in Molecular Biology, vol 1207. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1396-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-1396-1_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1395-4

  • Online ISBN: 978-1-4939-1396-1

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