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Galectins pp 201-213 | Cite as

Examination of Galectins in Phagocytosis

  • Huan-Yuan Chen
  • I-Chun Weng
  • Chi-Shan Li
  • Lei Wan
  • Fu-Tong LiuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1207)

Abstract

Galectins, a family of β-galactoside-binding proteins, are expressed in many different phagocytic leukocytes (granulocytes, monocytes, and macrophages). A number of family members have been shown to play an important role in ingestion of particles (phagocytosis), thus contributing to clearance of damaged cells and host defense against pathogens. Here we describe procedures for analysis of the roles of galectins in phagocytosis by using galectin-3 as an example. We emphasize the function of endogenous galectin-3 as determined by comparison of phagocytosis by macrophages from galectin-3 knockout mice and wild-type mice. We focus on the role of galectin-3 in phagocytosis of pathogens and Fcγ receptor-mediated phagocytosis of opsonized cells and particles.

Key words

Galectin Phagocytosis Opsonization Macrophage 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Huan-Yuan Chen
    • 1
    • 2
  • I-Chun Weng
    • 1
    • 2
  • Chi-Shan Li
    • 2
  • Lei Wan
    • 1
    • 4
  • Fu-Tong Liu
    • 3
    • 2
    Email author
  1. 1.Department of DermatologyUniversity of California Davis, School of MedicineSacramentoUSA
  2. 2.Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan
  3. 3.Department of DermatologyUniversity of California Davis, School of MedicineSacramentoUSA
  4. 4.School of Chinese MedicineChina Medical UniversityTaichungTaiwan

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