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Galectins pp 185-200 | Cite as

Detection of Phosphatidylserine Exposure on Leukocytes Following Treatment with Human Galectins

  • Connie M. Arthur
  • Lilian Cataldi Rodrigues
  • Marcelo Dias Baruffi
  • Harold C. Sullivan
  • Richard D. Cummings
  • Sean R. StowellEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1207)

Abstract

Cellular turnover represents a fundamental aspect of immunological homeostasis. While many factors appear to regulate leukocyte removal during inflammatory resolution, recent studies suggest that members of the galectin family play a unique role in orchestrating this process. Unlike cellular removal through apoptotic cell death, several members of the galectin family induce surface expression of phosphatidylserine (PS), a phagocytic marker on cells undergoing apoptosis, in the absence of cell death. However, similar to PS on cells undergoing apoptosis, galectin-induced PS exposure sensitizes cells to phagocytic removal. As galectins appear to prepare cells for phagocytic removal without actually inducing apoptotic cell death, this process has recently been coined preaparesis. Given the unique characteristics of galectin-induced PS exposure in the context of preaparesis, we will examine important considerations when evaluating the potential impact of different galectin family members on PS exposure and cell viability.

Key words

Galectin Phosphatidylserine (PS) Inflammatory resolution Leukocyte turnover Preaparesis 

Notes

Acknowledgments

This work was supported in part by grants from the National Blood Foundation, American Society of Hematology and Hemophilia of Georgia to S.R.S.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Connie M. Arthur
    • 1
  • Lilian Cataldi Rodrigues
    • 2
  • Marcelo Dias Baruffi
    • 2
  • Harold C. Sullivan
    • 1
  • Richard D. Cummings
    • 3
  • Sean R. Stowell
    • 4
    Email author
  1. 1.The Department of Pathology and Laboratory MedicineEmory University School of MedicineAtlantaUSA
  2. 2.Faculty of Pharmaceutical Sciences of Ribeirão Preto, Department of Clinical, Toxicological and Bromatological AnalysisUniversity of Sao PauloRibeirão Preto-SBrazil
  3. 3.Department of BiochemistryEmory University School of MedicineAtlantaUSA
  4. 4.Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory MedicineEmory University School of MedicineAtlantaUSA

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