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Galectins pp 153–167Cite as

Examination of the Role of Galectins in Plasma Cell Differentiation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1207))

Abstract

Plasma cells are terminally differentiated B cells that develop via the stimulation of mature B cells with various agents such as antigens and mitogens. Recently, we found that plasma cell differentiation can be modulated by galectin-1 and galectin-8; these galectins appear to play additive and redundant roles in promoting the production of antibody. Here, we describe the protocols for how to investigate the roles of galectins in plasma cell differentiation. These methods include the preparation of recombinant galectins from Escherichia coli for exogenously treating primary B cells, generation of galectin_Fcm fusion proteins for determining their binding to B cells, introduction of ectopic galectins in primary B cells using retroviral vectors, and inhibition of the binding of galectins to B cells by synthetic disaccharides.

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Authors and Affiliations

  1. Genomics Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang District, Taipei 115, Taiwan

    Chih-Ming Tsai & Kuo-I Lin

Authors
  1. Chih-Ming Tsai
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  2. Kuo-I Lin
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Correspondence to Kuo-I Lin .

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Editors and Affiliations

  1. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA

    Sean R. Stowell

  2. Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, Georgia, USA

    Richard D. Cummings

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Tsai, CM., Lin, KI. (2015). Examination of the Role of Galectins in Plasma Cell Differentiation. In: Stowell, S., Cummings, R. (eds) Galectins. Methods in Molecular Biology, vol 1207. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1396-1_10

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  • DOI: https://doi.org/10.1007/978-1-4939-1396-1_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1395-4

  • Online ISBN: 978-1-4939-1396-1

  • eBook Packages: Springer Protocols

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