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Sequence-Defined Oligoaminoamides for the Delivery of siRNAs

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Regulatory Non-Coding RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1206))

Abstract

Since it was found that synthetic small interfering RNA (siRNA) can invoke RNA interference (RNAi) responses in mammalian cells, it has gained enormous attention as a tool for gene silencing in basic science and as a novel therapeutic modality. To develop carriers for cytosolic and systemic siRNA delivery, our laboratory has recently developed a sequence-defined polymer platform compatible with solid-phase-supported synthesis. These polymers have displayed efficient siRNA-mediated gene silencing in vitro and in vivo. In this chapter, we provide a brief background on the special features of these polymers and detailed protocols to evaluate polyplex formation, gene silencing efficiency, and cytotoxicity of siRNA-containing polyplexes.

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Acknowledgements

This work was supported by the German Research Foundation grant SFB1032 (project B4) and the excellence cluster Nanosystems Initiative Munich. D.J.L. was supported by the Bavarian Research Foundation PhD Scholarship.

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Authors and Affiliations

  1. Department of Pharmacy and Center for Nanoscience (CeNS), Pharmaceutical Biotechnology, Center for System-Based Drug Research, Ludwig Maximilians University of Munich, Building D, Butenandtstraße 5-13, Munich, 81377, Germany

    Dian-Jang Lee, Ernst Wagner & Taavi Lehto

Authors
  1. Dian-Jang Lee
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  2. Ernst Wagner
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  3. Taavi Lehto
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Corresponding author

Correspondence to Taavi Lehto .

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Editors and Affiliations

  1. University of Connecticut Health Center, Farmington, Connecticut, USA

    Gordon G. Carmichael

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Lee, DJ., Wagner, E., Lehto, T. (2015). Sequence-Defined Oligoaminoamides for the Delivery of siRNAs. In: Carmichael, G. (eds) Regulatory Non-Coding RNAs. Methods in Molecular Biology, vol 1206. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1369-5_2

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  • DOI: https://doi.org/10.1007/978-1-4939-1369-5_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1368-8

  • Online ISBN: 978-1-4939-1369-5

  • eBook Packages: Springer Protocols

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