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Yeast Genetics pp 275–302Cite as

Analysis of Silencing in Saccharomyces cerevisiae

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1205))

Abstract

Silencing assays have proven to be powerful tools not only for understanding how epigenetic processes function and defining the structural components of silent chromatin, but also for a useful readout for characterizing the functions of proteins involved in chromatin biology that influence epigenetic processes directly or indirectly. This chapter describes a collection of assays for monitoring silencing in Saccharomyces cerevisiae, including qualitative and quantitative methods as well as protocols that provide either indirect or direct measurements of the transcriptional state of loci regulated by silent chromatin.

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Acknowledgements

This work was supported by the National Science Foundation (A.L.K.) and the Purdue University Center for Cancer Research (A.L.K.).

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Authors and Affiliations

  1. Department of Biochemistry and Purdue University Center for Cancer Research, Purdue University, 175 S. University St., West Lafayette, IN, 47907, USA

    Andrew Miller & Ann L. Kirchmaier

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  1. Andrew Miller
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  2. Ann L. Kirchmaier
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Corresponding author

Correspondence to Ann L. Kirchmaier .

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Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia, USA

    Jeffrey S. Smith

  2. Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia, USA

    Daniel J. Burke

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Miller, A., Kirchmaier, A.L. (2014). Analysis of Silencing in Saccharomyces cerevisiae . In: Smith, J., Burke, D. (eds) Yeast Genetics. Methods in Molecular Biology, vol 1205. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1363-3_17

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  • DOI: https://doi.org/10.1007/978-1-4939-1363-3_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1362-6

  • Online ISBN: 978-1-4939-1363-3

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