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Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS) for Mass Spectrometry Imaging of Small Molecules

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Mass Spectrometry Imaging of Small Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1203))

Abstract

Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS), a parallel technique to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), utilizes inorganic particles or porous surfaces to aid in the desorption/ionization of low-molecular-weight (MW) analytes. As a matrix-free and “soft” LDI approach, SALDI offers the benefit of reduced background noise in the low MW range, allowing for easier detection of biologically significant small MW species. Despite the inherent advantages of SALDI-MS, it has not reached comparable sensitivity levels to MALDI-MS. In relation to mass spectrometry imaging (MSI), intense efforts have been made in order to improve sensitivity and versatility of SALDI-MSI. We describe herein a detailed protocol that utilizes a hybrid LDI method, matrix-enhanced SALDI-MS (ME-SALDI MS), to detect and image low MW species in an imaging mode.

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Authors and Affiliations

  1. Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, CB 8204, Raleigh, NC, 27695, USA

    Victoria L. Brown, Qiang Liu & Lin He

Authors
  1. Victoria L. Brown
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  2. Qiang Liu
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  3. Lin He
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Corresponding author

Correspondence to Lin He .

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Editors and Affiliations

  1. Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA

    Lin He

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Brown, V.L., Liu, Q., He, L. (2015). Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS) for Mass Spectrometry Imaging of Small Molecules. In: He, L. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 1203. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1357-2_17

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  • DOI: https://doi.org/10.1007/978-1-4939-1357-2_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1356-5

  • Online ISBN: 978-1-4939-1357-2

  • eBook Packages: Springer Protocols

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