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Nanoparticle-Assisted Laser Desorption/Ionization for Metabolite Imaging

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Mass Spectrometry Imaging of Small Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1203))

Abstract

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) has enabled the spatial analysis of various molecules, including peptides, nucleic acids, lipids, and drug molecules. To expand the capabilities of MALDI-IMS, we have established an imaging technique using metal nanoparticles (NPs) to visualize metabolites, termed nanoparticle-assisted laser desorption/ionization imaging mass spectrometry (nano-PALDI-IMS). By utilizing Ag-, Fe-, Au-, and TiO2-derived NPs, we have succeeded in visualizing various metabolites, including fatty acid and glycosphingolipids, with higher sensitivity and spatial resolution than conventional techniques. Herein, we describe the practical experimental procedures and methods associated with nano-PALDI-IMS for the visualization of these molecules.

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Authors and Affiliations

  1. Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu-shi, Shizuoka, Japan

    Michihiko Waki, Eiji Sugiyama, Takeshi Kondo, Keigo Sano & Mitsutoshi Setou

Authors
  1. Michihiko Waki
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  2. Eiji Sugiyama
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  3. Takeshi Kondo
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  4. Keigo Sano
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  5. Mitsutoshi Setou
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Corresponding author

Correspondence to Mitsutoshi Setou .

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Editors and Affiliations

  1. Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA

    Lin He

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Waki, M., Sugiyama, E., Kondo, T., Sano, K., Setou, M. (2015). Nanoparticle-Assisted Laser Desorption/Ionization for Metabolite Imaging. In: He, L. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 1203. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1357-2_16

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  • DOI: https://doi.org/10.1007/978-1-4939-1357-2_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1356-5

  • Online ISBN: 978-1-4939-1357-2

  • eBook Packages: Springer Protocols

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