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Examining Estrogen Regulation of Cancer Stem Cells Through Multicolor Lineage Tracing

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Steroid Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1204))

Abstract

We have designed a dual-color fluorescent reporter that can track microRNA expression in vitro, which can be used for lineage tracing experiments. We have used this system to track miR-140 promoter activity in breast cancer cells and to follow the impact of estrogen signaling in cancer stem cell subpopulations.

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Acknowledgments

This work was supported by grants from ACS and NCI R01 (QZ).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Maryland Baltimore School of Medicine, Biomedical Research Facility, 337, Baltimore, MD, 21210, USA

    Yongshu Zhang, Gabriel Eades & Qun Zhou

Authors
  1. Yongshu Zhang
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  2. Gabriel Eades
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  3. Qun Zhou
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Corresponding author

Correspondence to Qun Zhou .

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Editors and Affiliations

  1. Second University of Naples, Naples, Italy

    Gabriella Castoria  & Ferdinando Auricchio  & 

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Zhang, Y., Eades, G., Zhou, Q. (2014). Examining Estrogen Regulation of Cancer Stem Cells Through Multicolor Lineage Tracing. In: Castoria, G., Auricchio, F. (eds) Steroid Receptors. Methods in Molecular Biology, vol 1204. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-1346-6_4

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  • DOI: https://doi.org/10.1007/978-1-4939-1346-6_4

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-1345-9

  • Online ISBN: 978-1-4939-1346-6

  • eBook Packages: Springer Protocols

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