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High-Performance Lectin Affinity Chromatography

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Lectins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1200))

Abstract

Lectin high-performance liquid chromatography techniques have contributed to the growing interest in glycoproteomics. Affinity chromatography is a very effective method to separate and purify trace amount of biological substances. In this chapter, we describe a basic procedure for separation of glycoproteins using commercially available lectin-HPLC columns. As an example, α-fetoprotein, known as a biomarker of liver cancer, can be separated at the level of their glyco-isomers by using a Lens culinaris agglutinin (LCA) column.

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References

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Authors and Affiliations

  1. J-Oil Mills, Inc., 11, Kagetoricho, Totsuka-ku, Yokohama, Kanagawa, 245-0064, Japan

    Yuka Kobayashi

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  1. Yuka Kobayashi
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Corresponding author

Correspondence to Yuka Kobayashi .

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Editors and Affiliations

  1. National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan

    Jun Hirabayashi

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© 2014 Springer Science+Business Media New York

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Kobayashi, Y. (2014). High-Performance Lectin Affinity Chromatography. In: Hirabayashi, J. (eds) Lectins. Methods in Molecular Biology, vol 1200. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1292-6_6

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  • DOI: https://doi.org/10.1007/978-1-4939-1292-6_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1291-9

  • Online ISBN: 978-1-4939-1292-6

  • eBook Packages: Springer Protocols

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