Abstract
Isothermal titration calorimetry (ITC) is a powerful tool for analyzing lectin–glycan interactions because it can measure the binding affinity and thermodynamic properties such as ∆H and ΔS in a single experiment without any chemical modification or immobilization. Here we describe a method for preparing glycan and lectin solution to minimize the buffer mismatch, setting parameters, and performing experiments.
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Takeda, Y., Matsuo, I. (2014). Isothermal Calorimetric Analysis of Lectin–Sugar Interaction. In: Hirabayashi, J. (eds) Lectins. Methods in Molecular Biology, vol 1200. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1292-6_18
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DOI: https://doi.org/10.1007/978-1-4939-1292-6_18
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