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Analysis of Quantum Dots and Their Conjugates by Capillary Electrophoresis with Detection of Laser-Induced Luminescence

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Quantum Dots: Applications in Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1199))

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Abstract

In many bioanalytical applications, important molecules such as DNA, proteins, and antibodies are routinely conjugated with fluorescent tags to reach an extraordinary sensitivity of analyses. Semiconductor nanoparticles, quantum dots, have already proved to be suitable components of highly luminescent tags, probes, and sensors with a broad applicability in analytical chemistry. Quantum dots provide high extinction coefficients together with a wide range of excitation wavelengths, size- and composition-tunable emissions, narrow and symmetric emission spectra, good quantum yields, relatively long size-dependent luminescence lifetime, and practically no photobleaching. Most of these properties are superior when compared with conventional organic fluorescent dyes. In this chapter, optimized procedures for the preparation of water-dispersed cadmium telluride (CdTe) quantum dots, conjugating reactions with antibodies, DNA, and macrocycles as well as their analyses by capillary electrophoresis are described. The potential of capillary electrophoresis for fast analyses of nanoparticles, their conjugates with antibodies, and immunocomplexes with targeted antigens is demonstrated on examples.

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Acknowledgement

This work was financially supported by grant of the Grant Agency of the Czech Republic 203/11/2377.

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Authors and Affiliations

  1. Institute of Analytical Chemistry, Czech Academy of Sciences, Veveří 97, 602 00, Brno, Czech Republic

    Karel Klepárník, Vladimíra Datinská, Ivona Voráčová & Marcela Lišková

Authors
  1. Karel Klepárník
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  2. Vladimíra Datinská
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  3. Ivona Voráčová
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  4. Marcela Lišková
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Corresponding author

Correspondence to Karel Klepárník .

Editor information

Editors and Affiliations

  1. Research Group on Biomedical Nanotechnology Biophysics and Radiobiology Department, Federal University of Pernambuco, Recife, Brazil

    Adriana Fontes

  2. Research Group on Biomedical Nanotechnology Pharmaceutical Sciences Department, Federal University of Pernambuco, Recife, Brazil

    Beate Saegesser Santos

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Klepárník, K., Datinská, V., Voráčová, I., Lišková, M. (2014). Analysis of Quantum Dots and Their Conjugates by Capillary Electrophoresis with Detection of Laser-Induced Luminescence. In: Fontes, A., Santos, B. (eds) Quantum Dots: Applications in Biology. Methods in Molecular Biology, vol 1199. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1280-3_3

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  • DOI: https://doi.org/10.1007/978-1-4939-1280-3_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1279-7

  • Online ISBN: 978-1-4939-1280-3

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