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Drosophila as a Model for Intestinal Infections

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Host-Bacteria Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1197))

Abstract

Drosophila melanogaster is a powerful model to study infections thanks to the power of its genetics and knowledge on its biology accumulated for over a century. While the systemic humoral immune response against invading microbes has been intensively studied in the past two decades, the study of intestinal infections is more recent. Here, we present the methods that are currently in use to probe various aspects of the host-pathogen interactions between Drosophila and ingested microbes, with an emphasis on the study of the midgut epithelium, which constitutes the major interface between the organism and the microbe-rich ingested food.

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Acknowledgements

We acknowledge the expert technical help from Mme Marie-Céline Lacombe and thank Mme Samantha Haller for advice. Work in the author’s laboratory has been funded by CNRS, ANR (DROSELEGANS, DROSOGUT, KANJI), Fondation Simone et Cino Del Duca, and Fondation pour la Recherche Médicale (Equipe FRM).

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    Authors and Affiliations

    1. UPR9022 du CNRS, Université de Strasbourg, 15, rue René Descartes, 67084, Strasbourg Cedex, France

      Matthieu Lestradet, Kwang-Zin Lee & Dominique Ferrandon

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    1. Matthieu Lestradet
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    2. Kwang-Zin Lee
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    3. Dominique Ferrandon
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    Correspondence to Dominique Ferrandon .

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    Editors and Affiliations

    1. U1047, UFR Médecine, INSERM, Université Montpellier 1, Nîmes, France

      Annette C. Vergunst

    2. U1047, UFR Médecine, INSERM, Université Montpellier 1, Nîmes, France

      David O'Callaghan

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    Lestradet, M., Lee, KZ., Ferrandon, D. (2014). Drosophila as a Model for Intestinal Infections. In: Vergunst, A., O'Callaghan, D. (eds) Host-Bacteria Interactions. Methods in Molecular Biology, vol 1197. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1261-2_2

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    • DOI: https://doi.org/10.1007/978-1-4939-1261-2_2

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-1260-5

    • Online ISBN: 978-1-4939-1261-2

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