Skip to main content
SpringerLink
Log in
Book cover

Mass Spectrometry in Metabolomics pp 75–80Cite as

Sample Preparation Methods for LC-MS-Based Global Aqueous Metabolite Profiling

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1198))

Abstract

Metabolite extraction is a key step in metabolomic analyses, particularly for untargeted studies. The extraction determines the types of metabolites that will be detected and the analytical platform to be used. In this chapter we describe two protocols aimed at detecting polar metabolites from biological samples; the first is aimed at detecting reduced species by LC/MS, and the second satisfies the requirements for both NMR and LC/MS analysis simultaneously.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Lin CY, Wu HF, Tjeerdema RS et al (2007) Evaluation of metabolite extraction strategies from tissue samples using NMR metabolomics. Metabolomics 3:55–67

    Article  CAS  Google Scholar 

  2. Okazaki Y, Saito K (2012) Recent advances of metabolomics in plant biotechnology. Plant Biotechnol Rep 6:1–15

    Article  PubMed  PubMed Central  Google Scholar 

  3. Wu HF, Southam AD, Hines A et al (2008) High-throughput tissue extraction protocol for NMR- and MS-based metabolomics. Anal Biochem 372:204–212

    Article  PubMed  CAS  Google Scholar 

  4. Beltran A, Suarez M, Rodriguez MA et al (2012) Assessment of compatibility between extraction methods for NMR- and LC/MS-based metabolomics. Anal Chem 84: 5838–5844

    Article  PubMed  CAS  Google Scholar 

  5. Moco S, Forshed J, De Vos RCH et al (2008) Intra- and inter-metabolite correlation spectroscopy of tomato metabolomics data obtained by liquid chromatography-mass spectrometry and nuclear magnetic resonance. Metabolomics 4:202–215

    Article  CAS  Google Scholar 

  6. Sellick CA, Hansen R, Stephens GM et al (2011) Metabolite extraction from suspension-cultured mammalian cells for global metabolite profiling. Nat Protoc 6:1241–1249

    Article  PubMed  CAS  Google Scholar 

  7. Yanes O, Tautenhahn R, Patti GJ et al (2011) Expanding coverage of the metabolome for global metabolite profiling. Anal Chem 83: 2152–2161

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  8. Lindon JC, Nicholson JK, Holmes E (2007) The handbook of metabonomics and metabolomics, 1st edn. Elsevier, Amsterdam, pp 79–86

    Google Scholar 

  9. Nicholson JK, Foxall PJD, Spraul M et al (1995) 750-MHz H-1 and H-1-C-13 NMR-spectroscopy of human blood-plasma. Anal Chem 67:793–811

    Article  PubMed  CAS  Google Scholar 

  10. Garrod S, Humpfer E, Spraul M et al (1999) High-resolution magic angle spinning H-1 NMR spectroscopic studies on intact rat renal cortex and medulla. Magn Reson Med 41:1108–1118

    Article  PubMed  CAS  Google Scholar 

  11. Genome Alberta Genome Canada. 2005–2013©. HMDB Version: 3.5. http://www.hmdb.ca Accessed May 15, 2013.

Download references

Author information

Authors and Affiliations

  1. Metabolomics Platform, Spanish Biomedical Research Center in Diabetes and Associated Metabolic Disorders (CIBERDEM), Barcelona, Spain

    Antoni Beltran, Sara Samino & Oscar Yanes

  2. Centre for Omic Sciences (COS), Rovira i Virgili University, Avinguda Universitat 3, Reus, 43204, Spain

    Antoni Beltran, Sara Samino & Oscar Yanes

  3. Institut d’Investigació Biomèdica Pere Virgili (IISPV), Reus, 43201, Spain

    Antoni Beltran, Sara Samino & Oscar Yanes

Authors
  1. Antoni Beltran
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sara Samino
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Oscar Yanes
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Oscar Yanes .

Editor information

Editors and Affiliations

  1. University of Washington Fred Hutchinson Cancer Rsrch Ctr, Seattle, Washington, USA

    Daniel Raftery

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media New York

About this protocol

Cite this protocol

Beltran, A., Samino, S., Yanes, O. (2014). Sample Preparation Methods for LC-MS-Based Global Aqueous Metabolite Profiling. In: Raftery, D. (eds) Mass Spectrometry in Metabolomics. Methods in Molecular Biology, vol 1198. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1258-2_5

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-1258-2_5

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1257-5

  • Online ISBN: 978-1-4939-1258-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation