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The Genetics of Murine Hox Loci: TAMERE, STRING, and PANTHERE to Engineer Chromosome Variants

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Hox Genes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1196))

Abstract

Following their duplications at the base of the vertebrate clade, Hox gene clusters underwent remarkable sub- and neo-functionalization events. Many of these evolutionary innovations can be associated with changes in the transcriptional regulation of their genes, where an intricate relationship between the structure of the gene cluster and the architecture of the surrounding genomic landscape is at play. Here, we report on a portfolio of in vivo genome engineering strategies in mice, which have been used to probe and decipher the genetic and molecular underpinnings of the complex regulatory mechanisms implemented at these loci.

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Acknowledgements

Patrick Tschopp is supported by postdoctoral fellowships from the Swiss National Science Foundation (SNF), EMBO, and the Human Frontiers Science Program (HFSP). Work in the Duboule laboratories is supported by the Ecole Polytechnique Fédérale de Lausanne, the University of Geneva, the National Center for Competence in Research Frontiers in Genetics, the Swiss National Research Fund, and the ERC grant SystemsHox.ch. We thank Bénédicte Mascrez for the organization of the genotyping pipeline and the supervision of mouse strain production.

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Authors and Affiliations

  1. Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA

    Patrick Tschopp

  2. National Research Centre ‘Frontiers in Genetics’, Department of Genetics and Evolution, University of Geneva, Sciences III, Quai Ernest-Ansermet 30, 1211, Geneva 4, Switzerland

    Denis Duboule

  3. School of Life Sciences, Federal Institute of Technology (EPFL), 1015, Lausanne, Switzerland

    Denis Duboule

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  1. Patrick Tschopp
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Correspondence to Denis Duboule .

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Editors and Affiliations

  1. IBDML, Marseille, France

    Yacine Graba

  2. Université Catholique de Louvain, Louvain-la-Neuve, Belgium

    René Rezsohazy

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Tschopp, P., Duboule, D. (2014). The Genetics of Murine Hox Loci: TAMERE, STRING, and PANTHERE to Engineer Chromosome Variants. In: Graba, Y., Rezsohazy, R. (eds) Hox Genes. Methods in Molecular Biology, vol 1196. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1242-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-1242-1_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1241-4

  • Online ISBN: 978-1-4939-1242-1

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