The Genetics of Murine Hox Loci: TAMERE, STRING, and PANTHERE to Engineer Chromosome Variants
Following their duplications at the base of the vertebrate clade, Hox gene clusters underwent remarkable sub- and neo-functionalization events. Many of these evolutionary innovations can be associated with changes in the transcriptional regulation of their genes, where an intricate relationship between the structure of the gene cluster and the architecture of the surrounding genomic landscape is at play. Here, we report on a portfolio of in vivo genome engineering strategies in mice, which have been used to probe and decipher the genetic and molecular underpinnings of the complex regulatory mechanisms implemented at these loci.
Key wordsMouse genetics Gene regulation In vivo genome engineering TAMERE STRING PANTHERE
Patrick Tschopp is supported by postdoctoral fellowships from the Swiss National Science Foundation (SNF), EMBO, and the Human Frontiers Science Program (HFSP). Work in the Duboule laboratories is supported by the Ecole Polytechnique Fédérale de Lausanne, the University of Geneva, the National Center for Competence in Research Frontiers in Genetics, the Swiss National Research Fund, and the ERC grant SystemsHox.ch. We thank Bénédicte Mascrez for the organization of the genotyping pipeline and the supervision of mouse strain production.
- 26.Vidal F, Sage J, Cuzin F, Rassoulzadegan M (1998) Cre expression in primary spermatocytes: a tool for genetic engineering of the germ line. Mol Reprod Dev 51:274–280. doi: 10.1002/(SICI)1098-2795(199811)51:3<274::AID-MRD6>3.0.CO;2-M PubMedCrossRefGoogle Scholar