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Hox Genes pp 59-87 | Cite as

Hox Complex Analysis Through BAC Recombineering

  • Mark Parrish
  • Youngwook Ahn
  • Christof Nolte
  • Bony De Kumar
  • Robb KrumlaufEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1196)

Abstract

BAC transgenesis in mice has proved to be useful in exploring the regulatory mechanisms and functions of the Hox complexes. The large constructs used may include most of the relevant components of the cis-regulatory landscape. Manipulations can be accomplished without compromising the integrity of the endogenous complex which reduces the likelihood of producing confounding phenotypic abnormalities. The development of recombineering tools has been critical in providing the means necessary to make many types of precise and varied manipulations of these large constructs. Here, we will discuss the methodologies necessary to manipulate Hox complex BACs, generation of transgenic animals bearing these constructs and the utilization of these resources to address fundamental aspects of Hox biology.

Key words

BAC Recombineering Hox Transgenic CRE Recombinase LoxP 

Notes

Acknowledgements

We thank members of the Krumlauf lab for valuable discussions, Heidi Monnin, Jenny Renyolds, and Andrea Moran and other members of the Stowers Laboratory Animal Services Facility for their care of our mice and technical assistance. All experiments involving mice were approved by the Institutional Animal Care and Use Committee of the Stowers Institute for Medical Research (Protocol 2010-0062/2013-0114). This research was supported by funds from the Stowers Institute and in addition Mark Parrish was funded in part by a Ruth L. Kirschstein NRSA Postdoctoral Fellowship (1F32GM075611-01/5F32GM075611-02).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Mark Parrish
    • 1
  • Youngwook Ahn
    • 1
  • Christof Nolte
    • 1
  • Bony De Kumar
    • 1
  • Robb Krumlauf
    • 1
    • 2
    Email author
  1. 1.Stowers Institute for Medical ResearchKansas CityUSA
  2. 2.Department of Anatomy and Cell BiologyKansas University Medical CenterKansas CityUSA

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