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Hox Genes pp 319-348 | Cite as

Hox Protein Interactions: Screening and Network Building

  • Isabelle Bergiers
  • Barbara Lambert
  • Sarah Daakour
  • Jean-Claude Twizere
  • René RezsohazyEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1196)

Abstract

Understanding the mode of action of Hox proteins requires the identification of molecular and cellular pathways they take part in. This includes to characterize the networks of protein-protein interactions involving Hox proteins. In this chapter we propose a strategy and methods to map Hox interaction networks, from yeast two-hybrid and high-throughput yeast two-hybrid interaction screening to bioinformatic analyses based on the software platform Cytoscape.

Key words

Hox proteins Yeast two-hybrid screening High-throughput yeast two-hybrid Affinity co-purification Interaction networks Cytoscape 

Notes

Acknowledgements

We are grateful to the Belgian Foundation against Cancer and to the Télévie program coordinated by the Belgian National Fund for Scientific Research (FNRS) for their support. I.B. and B.L. held a FRIA fellowship from the FNRS. S.D. is a “Leon Fredericq” fellow. J.C.T. is Research Investigator of the FNRS.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Isabelle Bergiers
    • 1
  • Barbara Lambert
    • 1
  • Sarah Daakour
    • 2
  • Jean-Claude Twizere
    • 2
  • René Rezsohazy
    • 1
    Email author
  1. 1.Institut des Sciences de la VieUniversité catholique de LouvainLouvain-la-NeuveBelgium
  2. 2.Laboratory of Signaling and Protein Interactions, GIGA-RUniversity of LiegeLiègeBelgium

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