Abstract
Understanding the mode of action of Hox proteins requires the identification of molecular and cellular pathways they take part in. This includes to characterize the networks of protein-protein interactions involving Hox proteins. In this chapter we propose a strategy and methods to map Hox interaction networks, from yeast two-hybrid and high-throughput yeast two-hybrid interaction screening to bioinformatic analyses based on the software platform Cytoscape.
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Acknowledgements
We are grateful to the Belgian Foundation against Cancer and to the Télévie program coordinated by the Belgian National Fund for Scientific Research (FNRS) for their support. I.B. and B.L. held a FRIA fellowship from the FNRS. S.D. is a “Leon Fredericq” fellow. J.C.T. is Research Investigator of the FNRS.
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Bergiers, I., Lambert, B., Daakour, S., Twizere, JC., Rezsohazy, R. (2014). Hox Protein Interactions: Screening and Network Building. In: Graba, Y., Rezsohazy, R. (eds) Hox Genes. Methods in Molecular Biology, vol 1196. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1242-1_20
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DOI: https://doi.org/10.1007/978-1-4939-1242-1_20
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