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Hox Genes pp 241-253 | Cite as

ChIP for Hox Proteins from Drosophila Imaginal Discs

  • Pavan Agrawal
  • L. S. ShashidharaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1196)

Abstract

Chromatin immunoprecipitation (ChIP) is a technique that reveals in vivo location of a protein bound to DNA. ChIP coupled with DNA microarrays (ChIP-chip) or next-generation sequencing (ChIP-seq) allows for identification of binding sites of transcription factors on a global scale. Here we describe a protocol for ChIP to identify binding of the Ultrabithorax (Ubx) Hox transcription factors from imaginal discs of Drosophila larvae. The protocol can be extended to other model organisms and transcription factors.

Key words

Drosophila Ubx Hox GAGA factor Chromatin immunoprecipitation Wing Haltere Imaginal discs 

Notes

Acknowledgements

The authors thank R Mishra, R White, Y Graba, and members of LSS and R Mishra lab for help with experiments and discussion. We thank Council of Scientific and Industrial Research, India, for a fellowship to PA and Department of Atomic Energy and Department of Science and Technology (Government of India) for research grants to LSS.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Indian Institute of Science Education & Research (IISER)Pashan, PuneIndia
  2. 2.Janelia Farm Research CampusHHMIAshburnUSA

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