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Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty

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Book cover Mouse Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1194))

Abstract

The ability to accurately model human cancer in mice enables in vivo examination of the biological mechanisms related to cancer initiation and progression as well as preclinical testing of new anticancer treatments and potential targets. The emergence of the genetically engineered Sleeping Beauty system of insertional mutagenesis has led to the development of a new generation of genetic mouse models of cancer and identification of novel cancer-causing genes. This chapter reviews the published cancer models of Sleeping Beauty and strategies using available strains to generate several models of cancer.

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Acknowledgements

V.M.H. is a Cancer Institute NSW South Wales (CINSW) Northern Translational Cancer Research Unit Fellow; E.K.C. is a CINSW Fellow. This work is supported by Cancer Council NSW and Cure Cancer Australia Foundation. Dr J. Grim and A. Knecht from the Fred Hutchinson Cancer Research Center are acknowledged for primer sequences for genotyping Rosa26SB11.

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Authors and Affiliations

  1. Bill Walsh Translational Cancer Research Laboratory, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, Level 8, Kolling Building, St Leonards, NSW, 2065, Australia

    Viive M. Howell & Emily K. Colvin

Authors
  1. Viive M. Howell
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  2. Emily K. Colvin
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Corresponding author

Correspondence to Viive M. Howell .

Editor information

Editors and Affiliations

  1. Stem Cell Regulation and Animal Aging Section, Basic Research Laboratory,, National Cancer Institute, National Institutes of Health, Frederick, MD, USA

    Shree Ram Singh

  2. Genetically Engineered Mouse Modeling Core/Department of Molecular Virology, Immunology and Medical Genetics, Wexner Medical Center, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA

    Vincenzo Coppola

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Howell, V.M., Colvin, E.K. (2014). Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty. In: Singh, S., Coppola, V. (eds) Mouse Genetics. Methods in Molecular Biology, vol 1194. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1215-5_21

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  • DOI: https://doi.org/10.1007/978-1-4939-1215-5_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1214-8

  • Online ISBN: 978-1-4939-1215-5

  • eBook Packages: Springer Protocols

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