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Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System

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Mouse Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1194))

Abstract

Long-term hematopoietic stem cells (LT-HSCs) have the ability to self-renew and differentiate into all blood cell lineages. Understanding the genetic networks that regulate LT-HSC function in the adult bone marrow requires inducible gene targeting and bone marrow transplantations. In this chapter we describe the use of the inducible Mx1-Cre mouse model to delete genes in LT-HSCs and methodologies for examining the function of LT-HSCs following deletion.

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Acknowledgements

This work was supported by the National Cancer Institute Grant CA143193 (to Y.D.) and Department of Pediatrics at USUHS.

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Authors and Affiliations

  1. Department of Pediatrics, Uniformed Services University of the Health Sciences, Bridge Road, Bethesda, MD, 20814, USA

    Kristbjorn Orri Gudmundsson, Kevin Oakley, Yufen Han & Yang Du

Authors
  1. Kristbjorn Orri Gudmundsson
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  2. Kevin Oakley
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  3. Yufen Han
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  4. Yang Du
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Corresponding author

Correspondence to Kristbjorn Orri Gudmundsson .

Editor information

Editors and Affiliations

  1. Stem Cell Regulation and Animal Aging Section, Basic Research Laboratory,, National Cancer Institute, National Institutes of Health, Frederick, MD, USA

    Shree Ram Singh

  2. Genetically Engineered Mouse Modeling Core/Department of Molecular Virology, Immunology and Medical Genetics, Wexner Medical Center, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA

    Vincenzo Coppola

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© 2014 Springer Science+Business Media New York

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Gudmundsson, K.O., Oakley, K., Han, Y., Du, Y. (2014). Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System. In: Singh, S., Coppola, V. (eds) Mouse Genetics. Methods in Molecular Biology, vol 1194. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1215-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-1215-5_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1214-8

  • Online ISBN: 978-1-4939-1215-5

  • eBook Packages: Springer Protocols

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