Abstract
Animal model of intestinal inflammation is essential for the understanding of the pathomechanisms of inflammatory bowel diseases like Crohn’s disease and ulcerative colitis. Typically, naïve T-cells were transferred into immunocompromised mice and develop gut inflammation because of inappropriate downregulation. Here, we describe the isolation, purification, and transfer of CD4+CD25− T-cells into recipients to obtain an experimental colitis model for the analysis of pathogenesis.
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Acknowledgements
This work was supported by DFG grants WE46561/1, WE4656/2-1, and ELAN M1-09.06.23.1.
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Weigmann, B. (2014). Induction of Colitis in Mice (T-Cell Transfer Model). In: Waisman, A., Becher, B. (eds) T-Helper Cells. Methods in Molecular Biology, vol 1193. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1212-4_14
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DOI: https://doi.org/10.1007/978-1-4939-1212-4_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1211-7
Online ISBN: 978-1-4939-1212-4
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