Abstract
Studying a tissue-specific mast cell can be of particular benefit given the heterogeneity that is known to exist among mast cells isolated or developed from different sources. Methods for isolating mast cells from a variety of tissues have been in existence for a number of years although, over time, these methodologies have been refined. We have had considerable experience studying mast cells isolated from human lung tissue. It is for this reason that, in this chapter, we provide detailed methods for the isolation and purification of human lung mast cells. However, it should be noted that the methods that are described in this chapter are generally applicable to the isolation of mast cells from different tissues and this will also be discussed.
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Acknowledgements
The authors are grateful to Mr. J. Rao, Mr. J. Edwards, Mr. D. Hopkinson, and Mr. A. Ahmed (Cardiothoracic Surgery) and Dr. S.K. Suvarna, Dr. P. Kitsanta, and Dr. J. Bury (Histopathology) at the Northern General Hospital, Sheffield, for their invaluable help in providing lung tissue specimens.
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Farrington, J., Seward, E.P., Peachell, P.T. (2014). Mast Cell Purification Protocols. In: Gibbs, B., Falcone, F. (eds) Basophils and Mast Cells. Methods in Molecular Biology, vol 1192. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1173-8_3
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DOI: https://doi.org/10.1007/978-1-4939-1173-8_3
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