Abstract
Here we describe how murine basophils can be detected in vivo by flow cytometry and immunofluorescence staining. Basophils constitute a homogeneous population of CD4−CD19−CD49b+IgE+ cells in flow cytometric analysis. When IgE levels are low one can also use anti-FcεRI or anti-CD200R3 antibodies instead of anti-IgE. For immunofluorescence staining we use an anti-Mcpt8 antibody since Mcpt8 is a specific marker for murine basophils. We describe how to prepare the tissue to cut cryo-sections and how to perform the staining using a tyramide-based amplification kit.
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This work was supported in part by an ERC starting grant (PAS_241506) from the European Union.
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Schwartz, C., Voehringer, D. (2014). Identification of Murine Basophils by Flow Cytometry and Histology. In: Gibbs, B., Falcone, F. (eds) Basophils and Mast Cells. Methods in Molecular Biology, vol 1192. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1173-8_17
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DOI: https://doi.org/10.1007/978-1-4939-1173-8_17
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