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Measuring Histamine and Cytokine Release from Basophils and Mast Cells

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Book cover Basophils and Mast Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1192))

Abstract

Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.

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Author information

Authors and Affiliations

  1. Allergy Clinic, Dept. 22, 1. floor, Gentofte Hospital, Niels Andersens vej 65, 2900, Hellerup, Denmark

    Bettina M. Jensen & Per S. Skov

  2. Department of Dermatology, Odense University Hospital, Odense, Denmark

    Sidsel Falkencrone

Authors
  1. Bettina M. Jensen
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  2. Sidsel Falkencrone
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  3. Per S. Skov
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Corresponding author

Correspondence to Bettina M. Jensen .

Editor information

Editors and Affiliations

  1. Medway School of Pharmacy, The University of Kent, Chatham Maritime, Kent, United Kingdom

    Bernhard F. Gibbs

  2. Division of Molecular and Cellular Science, The School of Pharmacy, The University of Nottingham, Nottingham, United Kingdom

    Franco H. Falcone

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Jensen, B.M., Falkencrone, S., Skov, P.S. (2014). Measuring Histamine and Cytokine Release from Basophils and Mast Cells. In: Gibbs, B., Falcone, F. (eds) Basophils and Mast Cells. Methods in Molecular Biology, vol 1192. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1173-8_10

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  • DOI: https://doi.org/10.1007/978-1-4939-1173-8_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1172-1

  • Online ISBN: 978-1-4939-1173-8

  • eBook Packages: Springer Protocols

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