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Ex Vivo Generation of Murine IL-10-Producing B Cells by Fusokines

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Regulatory B Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1190))

Abstract

Naïve B cells are crucial components of adaptive immunity. In addition to their capacity to produce immunoglobulins, a minor subset termed regulatory B cells or Bregs has been proven to modulate inflammation through the secretion of soluble mediators. The two main technical difficulties with their clinical use lie in their relatively low abundance in vivo and the scarcity of known methods for their ex vivo expansion. While studying the pharmacological properties of a novel bifunctional granulocyte macrophage-colony-stimulating factor (GM-CSF) and IL-15 fusion transgene (GIFT15) on unfractionated splenocytes in vitro, we observed that the GIFT15 fusokine had a remarkable and unprecedented effect on naïve B cells by converting them into suppressor cells of B-cell ontogeny (hereafter referred to as GIFT15 inducible (i)Bregs). Moreover, GIFT15 promoted iBreg proliferation. We present in this report a detailed protocol using the GIFT15 fusokine as a tool for the ex vivo generation of murine iBregs, which may serve as an immediate remedy to their abundance challenge in the clinic.

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Acknowledgments

This work was supported by the Canadian Institute of Health Research grant MOP-15017.

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Authors and Affiliations

  1. The Department of Pharmacology, Université de Montréal, 6128, Downtown Station, Montreal, QC, Canada, H3C 1J7

    Aurélie Tormo, Edouard Al-Chami & Moutih Rafei

  2. Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University, 1365B Clifton Road, Atlanta, GA, 30322, USA

    Jiusheng Deng

  3. Faculty of Medicine, Université Paris Descartes, 12 École de Médecine, 75270, Paris Cedex 06, France

    Sonia Ziouani

Authors
  1. Aurélie Tormo
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  2. Jiusheng Deng
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  3. Edouard Al-Chami
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  4. Sonia Ziouani
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  5. Moutih Rafei
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Corresponding author

Correspondence to Moutih Rafei .

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Editors and Affiliations

  1. Department of Medical and Biological Sciences, University of Udine, Udine, Italy

    Gaetano Vitale

  2. Department of Medical and Biological Sciences, University of Udine, Udine, Italy

    Francesca Mion

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Tormo, A., Deng, J., Al-Chami, E., Ziouani, S., Rafei, M. (2014). Ex Vivo Generation of Murine IL-10-Producing B Cells by Fusokines. In: Vitale, G., Mion, F. (eds) Regulatory B Cells. Methods in Molecular Biology, vol 1190. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1161-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-1161-5_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1160-8

  • Online ISBN: 978-1-4939-1161-5

  • eBook Packages: Springer Protocols

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